Kidney Disease Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health (NIH), Bethesda, MD, United States.
Kidney Disease Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health (NIH), Bethesda, MD, United States.
Cell Signal. 2020 Oct;74:109701. doi: 10.1016/j.cellsig.2020.109701. Epub 2020 Jul 7.
Systems-based, agnostic approaches focusing on transcriptomics data have been employed to understand the pathogenesis of polycystic kidney diseases (PKD). While multiple signaling pathways, including Wnt, mTOR and G-protein-coupled receptors, have been implicated in late stages of disease, there were few insights into the transcriptional cascade immediately downstream of Pkd1 inactivation. One of the consistent findings has been transcriptional evidence of dysregulated metabolic and cytoskeleton remodeling pathways. Recent technical developments, including bulk and single-cell RNA sequencing technologies and spatial transcriptomics, offer new angles to investigate PKD. In this article, we review what has been learned based on transcriptional approaches and consider future opportunities.
基于系统的、与具体方法无关的方法侧重于转录组学数据，用于了解多囊肾病 (PKD) 的发病机制。虽然包括 Wnt、mTOR 和 G 蛋白偶联受体在内的多个信号通路已被牵连到疾病的晚期，但对于 Pkd1 失活后立即发生的转录级联反应几乎没有深入了解。一致的发现之一是转录证据表明代谢和细胞骨架重塑途径失调。最近的技术发展，包括批量和单细胞 RNA 测序技术和空间转录组学，为研究 PKD 提供了新的角度。在本文中，我们根据转录方法回顾了已经学到的知识，并考虑了未来的机会。
