Los Agata, Ziuzina Dana, Van Cleynenbreugel Robin, Boehm Daniela, Bourke Paula
Environmental Sustainability and Health Institute, Technological University Dublin, Dublin 7, Ireland.
Faculty of Engineering Technology, Katholieke University Leuven, 9000 Ghent, Belgium.
Foods. 2020 Jul 8;9(7):898. doi: 10.3390/foods9070898.
Atmospheric cold plasma (ACP) is under investigation for an extensive range of biocontrol applications in food biosystems. However, the development of a novel intervention technology requires a thorough evaluation of the potential for negative effects and the implications for the human and animal food chains' safety. The evaluations were performed using a contained, high-voltage, dielectric barrier discharge plasma system. The cytotoxicity of two types of food models-a liquid model (wheat model medium (WMM)) vs. a solid model (wheat grain extract (WGE)) was compared in vitro using the mammalian cell line CHO-K1. The residual toxicity of ACP treatment of grains for food purposes was assessed using the invertebrate model , by feeding the beetles with flour produced from ACP-treated wheat grains. The cytotoxic effects and changes in the chemistry of the ACP-treated samples were more pronounced in samples treated in a liquid form as opposed to actual wheat grains. The feeding trial using demonstrated no negative impacts on the survivability or weight profiles of insects. Investigations into the interactions of plasma-generated species with secondary metabolites in the food matrices are necessary to ensure the safety of plasma for food applications.
大气冷等离子体（ACP）正在食品生物系统中的广泛生物防治应用方面接受研究。然而，一种新型干预技术的开发需要对负面影响的可能性以及对人类和动物食物链安全的影响进行全面评估。评估是使用一个密闭的高压介质阻挡放电等离子体系统进行的。使用哺乳动物细胞系CHO-K1在体外比较了两种类型食品模型——液体模型（小麦模型培养基（WMM））与固体模型（小麦籽粒提取物（WGE））的细胞毒性。通过用经ACP处理的小麦籽粒生产的面粉喂养甲虫，使用无脊椎动物模型评估了用于食品目的的谷物经ACP处理后的残留毒性。与实际小麦籽粒相比，以液体形式处理的样品中，ACP处理样品的细胞毒性作用和化学变化更为明显。使用[此处原文缺失相关内容]进行的喂养试验表明，对昆虫的生存能力或体重状况没有负面影响。有必要研究等离子体产生的物质与食品基质中次生代谢物的相互作用，以确保等离子体在食品应用中的安全性。
