Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Molecules. 2021 Jun 6;26(11):3449. doi: 10.3390/molecules26113449.
is a multifunctional deciduous tree that is both a food and a source of traditional Chinese medicine for both humans and animals. Further analysis of the UGT gene family is of great significance to the utilization of . The substrates of plant genes include highly diverse and complex chemicals, such as flavonoids and terpenes. In order to deepen our understanding of this family, a comprehensive analysis was performed. Phylogenetic analysis showed that 155 were divided into 15 subgroups. A conserved motif analysis showed that proteins in the same subgroups possessed similar motif structures. Tandem duplication was the primary driving force for the expansion of the gene family. The global promoter analysis indicated that they were associated with complex hormone regulatory networks and the stress response, as well as the synthesis of secondary metabolites. The expression pattern analysis showed that the expression level of in leaves and roots was higher than that in fruits and stems. Next, we determined the composition and content of flavonoids, the main products of the reaction. A total of 19 compounds were isolated and analyzed by UPLC-ESI-MS/MS in 3 species of including , , and , and the number of compounds was different in these 3 species. The total flavonoid content and antioxidant capacities of the three species were analyzed respectively. All assays exhibited the same trend: the hybrid paper mulberry showed a higher total flavonoid content, a higher total phenol content and higher antioxidant activity than the other two species. Overall, our study provides valuable information for understanding the function of in the biosynthesis of flavonoids.
是一种多功能落叶乔木，既是人类和动物的食物，也是传统中药的来源。进一步分析 UGT 基因家族对 的利用具有重要意义。植物 UGT 基因的底物包括高度多样化和复杂的化学物质，如类黄酮和萜类化合物。为了加深对该家族的了解，进行了全面的分析。系统发育分析表明，155 个 UGT 基因被分为 15 个亚组。保守基序分析表明，同一亚组中的 UGT 蛋白具有相似的基序结构。串联重复是 UGT 基因家族扩张的主要驱动力。全局启动子分析表明，它们与复杂的激素调控网络和应激反应以及次生代谢物的合成有关。表达模式分析表明，UGT 在叶片和根中的表达水平高于在果实和茎中的表达水平。接下来，我们确定了 UGT 反应的主要产物——类黄酮的组成和含量。通过 UPLC-ESI-MS/MS 在 3 种 中分离和分析了 19 种化合物，包括 、 和 ，这 3 种植物中的化合物数量不同。分析了这 3 个物种的总黄酮含量和抗氧化能力。所有的测定都表现出相同的趋势：杂交构树表现出比其他两种更高的总黄酮含量、总酚含量和更高的抗氧化活性。总的来说，我们的研究为了解 UGT 在类黄酮生物合成中的功能提供了有价值的信息。
