Matsumoto K, Dowling J, Atkins R C
Department of Nephrology, Prince Henry's Hospital, Melbourne, Australia.
Am J Nephrol. 1988;8(6):463-70. doi: 10.1159/000167656.
Interleukin 1 (IL-1) activity was measured in glomerular culture supernatants from 3 patients with rapidly progressive crescentic glomerulonephritis (RPGN). Macrophages were present in both capillary tufts and cellular crescents as identified by OKM1-positive cells on immunoperoxidase labelling. Glomeruli from 4 rejecting renal cadaver allografts were used as a disease control, in addition to glomeruli from a normal kidney. IL-1 activity as measured by the thymocyte proliferation assay was greater in the supernatants from cultured glomerular outgrowths of patients with crescentic GN than in those from rejected renal allografts and glomeruli isolated from the normal tissue. IL-1 production from cultured glomerular cells from patients with RPGN was detectable in the serum-free conditioned media harvested after 3 days of culture and increased in a stepwise fashion over 28 days of culture. The prominent feature of the glomerular outgrowth of the glomeruli in the RPGN patients was the presence of large numbers of macrophages, which were not present in cultured control glomeruli. These findings indicate that the immunoregulatory aberration in patients with RPGN may in part be due to IL-1 production by activated macrophages.
对3例快速进行性新月体性肾小球肾炎（RPGN）患者的肾小球培养上清液中的白细胞介素1（IL-1）活性进行了测定。免疫过氧化物酶标记显示OKM1阳性细胞，毛细血管襻和细胞性新月体中均存在巨噬细胞。除了正常肾脏的肾小球外，还使用了4个正在发生排斥反应的肾移植尸体供肾的肾小球作为疾病对照。通过胸腺细胞增殖试验测定，新月体性肾小球肾炎患者培养的肾小球生长物上清液中的IL-1活性高于排斥肾移植和从正常组织分离的肾小球中的IL-1活性。RPGN患者培养的肾小球细胞在培养3天后收获的无血清条件培养基中可检测到IL-1产生，并在28天的培养过程中逐步增加。RPGN患者肾小球生长的突出特征是存在大量巨噬细胞，而培养的对照肾小球中不存在巨噬细胞。这些发现表明，RPGN患者的免疫调节异常可能部分归因于活化巨噬细胞产生的IL-1。
