Atkins R C, Glasgow E F, Holdsworth S R, Thomson N M, Hancock W W
Kidney Int. 1980 Apr;17(4):515-27. doi: 10.1038/ki.1980.60.
To study aspects of glomerular cell response in varying types of glomerulonephritis, we evaluated isolated glomeruli from 101 normal and diseased adult human renal biopsy specimens in tissue culture. Glomerular cell outgrowths from normal glomeruli consisted of large stellate cells (type I) and smaller fusiform cells (type II). A very occasional cell (type III), which had the features of a macrophage, was seen. The cellular outgrowth from isolated glomeruli reflected the numbers and types of cells present in the diseased glomeruli. Reduced numbers of cells were found in the outgrowth of sclerotic, hypocellular glomeruli. Glomeruli from biopsy specimens with altered morphologic appearances but no increase in cellularity (minimal change nephropathy, membranous nephropathy) produced the same growth pattern as from glomeruli isolated from normal kidneys. In mesangial proliferative nephritis, increased numbers of type II cells were detected in the outgrowth. Large numbers of macrophages were found in the glomerular outgrowth of patients with crescentic glomerulonephritis. Macrophages were absent when crescentic glomeruli were sclerosed, indicating the importance of macrophages in the acute hypercellular phase of crescent formation.
为研究不同类型肾小球肾炎中肾小球细胞反应的各个方面，我们在组织培养中评估了取自101例正常和患病成年人类肾活检标本的分离肾小球。正常肾小球的肾小球细胞生长物由大型星状细胞（I型）和较小的梭形细胞（II型）组成。偶尔可见具有巨噬细胞特征的细胞（III型）。分离肾小球的细胞生长反映了患病肾小球中存在的细胞数量和类型。在硬化性、细胞减少性肾小球的生长物中发现细胞数量减少。形态外观改变但细胞数量未增加的活检标本（微小病变肾病、膜性肾病）的肾小球产生的生长模式与从正常肾脏分离的肾小球相同。在系膜增生性肾炎中，生长物中检测到II型细胞数量增加。新月体性肾小球肾炎患者的肾小球生长物中发现大量巨噬细胞。当新月体性肾小球硬化时巨噬细胞消失，这表明巨噬细胞在新月体形成的急性高细胞期的重要性。
