Holdsworth S R, Thomson N M, Glasgow E F, Dowling J P, Atkins R C
J Exp Med. 1978 Jan 1;147(1):98-109. doi: 10.1084/jem.147.1.98.
As a means of studying mechanisms of response to injury in glomerulonephritis, glomeruli from normal sheep and rabbits and from sheep and rabbits with experimental crescentic glomerulonephritis have been isolated and grown in tissue culture. The cellular outgrowths from the normal and diseased glomeruli have been compared. The outgrowth of glomeruli from normal animals contained only two cell populations whose microscopic and ultrastructural appearances were of epithelial and mesangial cells. The same cells were also observed in the outgrowths of glomeruli from animals with crescenti nephritis but in addition a third population of cells was present in large numbers. These cells were identified as macrophages by their mobility, ultrastructure, phagocytic capacity, and presence of Fc receptors. Glomerular outgrowth from sheep with crescentic glomerulonephritis contained 170 +/- 20 (SEM) macrophages and outgrowths from rabbits with crescentic nephritis contained 64 +/- 6 (SEM) macrophages per glomerulus. We have previously observed large numbers of macrophages in the outgrowth of isolated glomeruli from humans with rapidly progressive crescentic glomerulonephritis. The predominance of the macrophage in cultures of glomeruli from both human and animal crescentic glomerulonephritis suggests that this is an important cell in the inflammatory reaction occurring in crescentic glomerulonephritis and may comprise a substantial proportion of the cells forming the crescent.
作为研究肾小球肾炎损伤反应机制的一种手段，已从正常绵羊和兔子以及患有实验性新月体性肾小球肾炎的绵羊和兔子中分离出肾小球，并在组织培养中进行培养。对正常和患病肾小球的细胞生长情况进行了比较。正常动物肾小球的生长物仅包含两个细胞群体，其微观和超微结构表现为上皮细胞和系膜细胞。在患有新月体性肾炎动物的肾小球生长物中也观察到了相同的细胞，但此外还大量存在第三种细胞群体。通过其移动性、超微结构、吞噬能力和Fc受体的存在，这些细胞被鉴定为巨噬细胞。患有新月体性肾小球肾炎的绵羊的肾小球生长物中每个肾小球含有170±20（标准误）个巨噬细胞，患有新月体性肾炎的兔子的肾小球生长物中每个肾小球含有64±6（标准误）个巨噬细胞。我们之前在患有快速进展性新月体性肾小球肾炎的人类分离肾小球的生长物中观察到大量巨噬细胞。在人类和动物新月体性肾小球肾炎的肾小球培养物中巨噬细胞占主导地位，这表明巨噬细胞是新月体性肾小球肾炎炎症反应中的重要细胞，并且可能占形成新月体的细胞的很大比例。
