Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Domest Anim Endocrinol. 2021 Jan;74:106498. doi: 10.1016/j.domaniend.2020.106498. Epub 2020 Jun 11.
Oxytocin is a hormone that is increasingly being used for welfare evaluation in animals. Although several types of samples have been used for oxytocin measurement, saliva can be a suitable option for pigs producing less stress than blood sampling. In this study, 3 different methods for oxytocin measurements, 2 based on alphaLISA technology (one with a monoclonal and other with a polyclonal antibody) and one commercially available kit, were compared in saliva of pigs. These methods were used in saliva samples obtained from female pigs at 3 different days during gestation and lactation, with and without a reduction/alkylation (R/A), which is a procedure for breaking the links between oxytocin and proteins of the sample. The assays showed a different behavior after the R/A procedure, with no significant changes in the oxytocin results in case of the alphaLISA monoclonal method, a significant decrease with the alphaLISA polyclonal method, and a significant increase with the commercial kit. Although all assays showed a similar tendency in detecting the changes in oxytocin during gestation and lactation, they showed changes of different magnitude and statistical signification. This report indicates that different assays can measure different forms of oxytocin present in saliva and can have a different behavior after R/A of the sample and when are used to measure oxytocin in gestation and lactation.
催产素是一种越来越多地用于动物福利评估的激素。尽管已经使用了几种类型的样本进行催产素测量，但与采血相比，唾液可以是一种产生较少应激的猪的合适选择。在这项研究中，比较了 3 种不同的催产素测量方法，其中 2 种基于 alphaLISA 技术（一种使用单克隆抗体，另一种使用多克隆抗体）和一种市售试剂盒，用于测量妊娠和哺乳期母猪的唾液样本。这些方法用于在妊娠和哺乳期的 3 天内获得的母猪唾液样本中，有和没有还原/烷基化（R/A），这是一种打破催产素与样品中蛋白质之间联系的程序。该分析方法在 R/A 后表现出不同的行为，在 alphaLISA 单克隆方法的情况下，催产素结果没有显著变化，在 alphaLISA 多克隆方法的情况下，催产素结果显著降低，在商业试剂盒的情况下，催产素结果显著增加。尽管所有分析方法在检测妊娠和哺乳期期间催产素的变化时都表现出相似的趋势，但它们表现出的变化幅度和统计学意义不同。本报告表明，不同的分析方法可以测量唾液中存在的不同形式的催产素，并且在对样品进行 R/A 后以及在用于测量妊娠和哺乳期期间的催产素时，它们的行为可能不同。
