Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain.
Department of Animal and Food Science, School of Veterinary Science, Universitat Autònoma de Barcelona, Barcelona, Spain.
Domest Anim Endocrinol. 2020 Jan;70:106384. doi: 10.1016/j.domaniend.2019.106384. Epub 2019 Aug 8.
Oxytocin is a hormone of interest in reproduction, but also in the field of psychology and behavior, being considered as a biomarker of positive emotions. Saliva can be a noninvasive way to measure oxytocin, which is very useful in species such as the pig where blood collection can produce a high degree of stress. In this study, a new assay for oxytocin measurement was developed, analytically validated, and used to measure possible changes in oxytocin in saliva of female pigs at different days after farrowing. The assay showed an adequate accuracy and precision and does not need a previous extraction step. In addition, oxytocin concentrations were significantly higher at day 1 of lactation than at day 9 after farrowing, but levels increased at day 20 again. This assay can contribute to a wider use of oxytocin measurements in pigs as it is a noninvasive sampling procedure that minimizes stress.
催产素是一种与生殖相关的激素，但也在心理学和行为学领域引起关注，被认为是积极情绪的生物标志物。唾液可以作为一种非侵入性的方法来测量催产素，这在猪等物种中非常有用，因为采血会产生高度的应激。在这项研究中，开发了一种新的催产素测量分析方法，并用于测量不同产仔后天数母猪唾液中催产素的可能变化。该方法具有足够的准确性和精密度，并且不需要进行先前的提取步骤。此外，泌乳第 1 天的催产素浓度明显高于产仔后第 9 天，但在第 20 天再次升高。该方法可以促进在猪中更广泛地使用催产素测量，因为它是一种非侵入性的采样程序，可以最大限度地减少应激。
