Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
Department of Animal Production, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Res Vet Sci. 2021 Dec;141:110-115. doi: 10.1016/j.rvsc.2021.10.013. Epub 2021 Oct 20.
This study aimed to evaluate whether insulin could be measured in the saliva of pigs and if its concentration changes in some physiological conditions. For this purpose, a validation of an automated heterologous immunoassay for measuring insulin in the saliva of pigs was performed. In addition, the possible changes of salivary insulin concentration in sows after food intake and during gestation and lactation were studied. The evaluated immunoassay was able to detect insulin in the saliva of pigs in a precise and accurate way when species-specific calibrators were used. There was no correlation in insulin concentrations between serum and saliva. Insulin concentrations showed a significant increase in the saliva of sows after feeding. Sows at farrowing and lactation presented higher salivary insulin levels as compared with those in gestation. In conclusion, the results showed that insulin could be measured in the saliva of pigs, and changes in its concentration can be detected due to food intake and different physiological conditions.
本研究旨在评估胰岛素是否可以在猪的唾液中被测量，以及其浓度是否会在某些生理条件下发生变化。为此，我们对一种用于测量猪唾液中胰岛素的自动化异源免疫分析方法进行了验证。此外，我们还研究了在进食后以及在妊娠和哺乳期间，母猪唾液中胰岛素浓度的可能变化。当使用物种特异性校准器时，所评估的免疫分析法能够精确且准确地检测猪唾液中的胰岛素。血清和唾液中的胰岛素浓度之间没有相关性。母猪进食后，唾液中的胰岛素浓度显著增加。分娩和哺乳期的母猪的唾液胰岛素水平明显高于妊娠期的母猪。总之，研究结果表明，胰岛素可以在猪的唾液中被测量，并且可以检测到其浓度因进食和不同的生理条件而发生的变化。
