Zhu Xingjie, Mou Changling, Zhang Fulin, Huang Yunshuai, Yang Chunyan, Ji Jingli, Liu Xi, Cao Penghui, Nguyen Thanhliem, Lan Jie, Zhou Chunlei, Liu Shijia, Jiang Ling, Wan Jianmin
State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
Department of Biology and Agricultural Engineering, Quynhon University, Quynhon, Binhdinh, 590000, Vietnam.
Rice (N Y). 2020 Jul 11;13(1):45. doi: 10.1186/s12284-020-00407-2.
The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete.
A new temperature-sensitive white stripe leaf 9(wsl9) rice mutant is described. The mutant develops white stripes during early leaf development, but becomes green after the three-leaf stage under field conditions. The wsl9 mutant was albinic when grown at low temperature. Gene mapping of the WSL9 locus, together with complementation tests indicated that WSL9 encodes a novel protein with an HNH domain. WSL9 was expressed in various tissues. Under low temperature, the wsl9 mutation caused defects in splicing of rpl2, but increased the editing efficiency of rpoB. Expression levels of plastid genome-encoded genes, which are transcribed by plastid-coded RNA polymerase (PEP), chloroplast development genes and photosynthesis-related genes were altered in the wsl9 mutant.
WSL9 encodes an HNH endonuclease domain-containing protein that is essential for early chloroplast development. Our study provides opportunities for further research on regulatory mechanisms of chloroplast development in rice.
植物叶绿体对于光合作用和其他细胞过程至关重要，但对植物叶绿体发育的生物学机制的了解尚不完整。
描述了一种新的温度敏感型白条纹叶9（wsl9）水稻突变体。该突变体在叶片发育早期出现白色条纹，但在田间条件下三叶期后变为绿色。wsl9突变体在低温下生长时呈白化状态。对WSL9基因座进行基因定位并结合互补试验表明，WSL9编码一种具有HNH结构域的新型蛋白质。WSL9在多种组织中表达。在低温下，wsl9突变导致rpl2剪接缺陷，但提高了rpoB的编辑效率。wsl9突变体中，由质体编码的RNA聚合酶（PEP）转录的质体基因组编码基因、叶绿体发育基因和光合作用相关基因的表达水平发生了改变。
WSL9编码一种含HNH核酸内切酶结构域的蛋白质，该蛋白质对早期叶绿体发育至关重要。我们的研究为进一步研究水稻叶绿体发育的调控机制提供了机会。
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