Fan Huanhuan, McGhee Claire E, Lake Ryan J, Yang Zhenglin, Guo Zijian, Zhang Xiao-Bing, Lu Yi
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
JACS Au. 2023 May 15;3(6):1615-1622. doi: 10.1021/jacsau.3c00062. eCollection 2023 Jun 26.
Manganese is an essential trace element in the human body that acts as a cofactor in many enzymes and metabolisms. It is important to develop methods to detect Mn in living cells. While fluorescent sensors have been very effective in detecting other metal ions, Mn-specific fluorescent sensors are rarely reported due to nonspecific fluorescence quenching by the paramagnetism of Mn and poor selectivity against other metal ions such as Ca and Mg. To address these issues, we herein report in vitro selection of an RNA-cleaving DNAzyme with exceptionally high selectivity for Mn. Through converting it into a fluorescent sensor using a catalytic beacon approach, Mn sensing in immune cells and tumor cells has been achieved. The sensor is also used to monitor degradation of manganese-based nanomaterials such as MnOx in tumor cells. Therefore, this work provides an excellent tool to detect Mn in biological systems and monitor the Mn-involved immune response and antitumor therapy.
锰是人体必需的微量元素，在许多酶和代谢过程中作为辅助因子发挥作用。开发检测活细胞中锰的方法很重要。虽然荧光传感器在检测其他金属离子方面非常有效，但由于锰的顺磁性导致的非特异性荧光猝灭以及对钙和镁等其他金属离子的选择性较差，很少有报道针对锰的特异性荧光传感器。为了解决这些问题，我们在此报告了一种对锰具有极高选择性的RNA切割脱氧核酶的体外筛选。通过使用催化信标方法将其转化为荧光传感器，实现了在免疫细胞和肿瘤细胞中对锰的检测。该传感器还用于监测肿瘤细胞中锰基纳米材料（如氧化锰）的降解。因此，这项工作为检测生物系统中的锰以及监测涉及锰的免疫反应和抗肿瘤治疗提供了一个出色的工具。
