College of Life Science, Northwest Normal University, 967 East Anning Road, 730070, Lanzhou, China.
Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Road, 518055, Shenzhen, China.
J Antibiot (Tokyo). 2020 Dec;73(12):863-867. doi: 10.1038/s41429-020-0347-9. Epub 2020 Jul 13.
A marine-sediment-derived bacterium Bacillus velezensis SH-B74 can produce cyclic lipopeptides (CLPs). This study presented the isolation, characterization, and activity evaluation of a new CLP from the bacterial cultures of the strain SH-B74. Multiple chromatographic methods (solid-phase extraction and reversed-phase high-performance liquid chromatography) were applied to the purifying procedure of CLP, and the structural characterization of the new CLP was conducted by various spectroscopy (1D and 2D nuclear magnetic resonance together with Fourier transform infrared spectroscopy) and spectrometry (liquid chromatography-mass spectrometry, high-resolution mass spectrometry and tandem mass spectrometry) techniques as well as Marfey's method. The results displayed that the new CLP (anteiso-C Ile surfactin, 1) consists of a peptidic backbone of L-Glu, L-Ile, D-Leu, L-Val, L-Asp, D-Leu, L-Ile, and an anteiso-C type saturated fatty acid chain. Further activity assay showed that the new CLP displays activity on the inhibition of the appressoria formation of rice blast causal pathogen Magnaporthe oryzae. To sum up, the results presented the perspective of potential application of the new CLP as a green agrichemical to control M. oryzae.
一株海洋沉积物来源的芽孢杆菌 Bacillus velezensis SH-B74 能够产生环脂肽 (CLP)。本研究从菌株 SH-B74 的细菌培养物中分离、鉴定并评价了一种新型 CLP。采用多种色谱方法（固相萃取和反相高效液相色谱）对 CLP 进行纯化，通过各种光谱（一维和二维核磁共振以及傅里叶变换红外光谱）和光谱技术（液相色谱-质谱、高分辨率质谱和串联质谱）以及 Marfey 法对新型 CLP 的结构进行了鉴定。结果表明，新型 CLP（anteiso-C Ile 表面活性素，1）由 L-Glu、L-Ile、D-Leu、L-Val、L-Asp、D-Leu、L-Ile 和 anteiso-C 型饱和脂肪酸链组成。进一步的活性测定表明，新型 CLP 对稻瘟病病原菌稻瘟病菌附着孢形成具有抑制活性。综上所述，该研究结果表明，新型 CLP 作为一种绿色农用化学品控制 M. oryzae 具有潜在的应用前景。
