Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
J Antibiot (Tokyo). 2012 Jun;65(6):317-22. doi: 10.1038/ja.2012.19. Epub 2012 Apr 11.
Three lipopeptides were isolated by bioactivity-guided fractionation from the fermentation broth of Bacillus mojavensis B0621A. A new iturinic lipopeptide, named mojavensin A, was tentatively characterized by 1D, 2D NMR and MS spectroscopy, Marfey's method containing a novel peptide backbone of L-Asn₁, D-Tyr₂, D-Asn₃, L-Gln₄, L-Pro₅, D-Asn₆, L-Asn₇ and an anteiso-type of the saturated β-fatty acid side chain. Compound 2 and 3 were tentatively identified as iso-C16 fengycin B and anteiso-C17 fengycin B, respectively. These lipopeptides displayed dose-dependent antifungal activity against a broad spectra of phytopathogens and were weakly antagonistic to Staphylococcus aureus. Moreover, they all revealed cytotoxic activities against the human leukemia (HL-60) cell line. Mojavensin A, iso-C16 fengycin B, and anteiso-C17 fengycin B inhibited the growth of HL-60 with IC₅₀ of 100, 100 and 1.6 μM, respectively.
从地衣芽孢杆菌 B0621A 的发酵液中，通过活性导向分离得到了 3 种脂肽。一种新的伊枯草菌素脂肽，命名为莫哈维森 A，通过 1D、2D NMR 和 MS 光谱、Marfey 法进行了初步表征，其中含有新型肽骨架 L-Asn₁、D-Tyr₂、D-Asn₃、L-Gln₄、L-Pro₅、D-Asn₆、L-Asn₇ 和饱和β-脂肪酸侧链的 anteiso 型。化合物 2 和 3 被初步鉴定为 iso-C16 丰原素 B 和 anteiso-C17 丰原素 B。这些脂肽对广谱植物病原菌表现出剂量依赖性的抗真菌活性，对金黄色葡萄球菌的拮抗作用较弱。此外，它们对人白血病（HL-60）细胞系均表现出细胞毒性活性。莫哈维森 A、iso-C16 丰原素 B 和 anteiso-C17 丰原素 B 对 HL-60 的生长抑制作用的 IC₅₀分别为 100、100 和 1.6 μM。
