Singh Nimisha, Millot Nadine, Maurizi Lionel, Lizard Gérard, Kumar Rajender
Department of Applied Chemistry, S. V. National Institute of Technology, Surat 395007, Gujarat, India.
Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS/Université Bourgogne Franche-Comté, 9 Av. A. Savary, BP 47 870, Dijon 21078, France.
ACS Omega. 2020 Jun 23;5(26):16165-16175. doi: 10.1021/acsomega.0c01747. eCollection 2020 Jul 7.
Multifunctional iron oxide magnetic nanoparticles, among them nanorods, were prepared with a mussel-inspired polydopamine (pDA) surface coating agent for cancer therapeutics. Taurine, a free sulfur-containing ß amino acid, was grafted on the pDA at the iron oxide nanoparticle surface to enhance its biocompatibility and targeted delivery action. Doxorubicin (DOX), an anticancer drug, was loaded on the prepared nanovehicles with an entrapment efficiency of 70.1%. Drug release kinetics were then analyzed using UV-vis and fluorescence spectroscopies, suggesting the pH-responsive behavior of the developed nanovehicle. The developed system was then tested on PC-3 cell lines to check its cellular response. Confocal microscopy observations and (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2-tetrazolium) and Annexin V-FITC assays used to evaluate cell toxicity and apoptosis reveal a dose-dependent nature of nanorods and can overcome the side effects of using free DOX with a targeted action.
采用受贻贝启发的聚多巴胺（pDA）表面涂层剂制备了多功能氧化铁磁性纳米颗粒，其中包括纳米棒，用于癌症治疗。牛磺酸是一种含游离硫的β氨基酸，被接枝到氧化铁纳米颗粒表面的pDA上，以增强其生物相容性和靶向递送作用。将抗癌药物阿霉素（DOX）负载到制备的纳米载体上，包封率为70.1%。然后使用紫外-可见光谱和荧光光谱分析药物释放动力学，表明所开发的纳米载体具有pH响应行为。然后在PC-3细胞系上测试所开发的系统，以检查其细胞反应。用于评估细胞毒性和凋亡的共聚焦显微镜观察以及（3-（4,5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2-四氮唑）和膜联蛋白V-异硫氰酸荧光素分析显示纳米棒具有剂量依赖性，并且可以通过靶向作用克服使用游离DOX的副作用。
