Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Department of Oral & Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Clin Oral Investig. 2021 Mar;25(3):1441-1450. doi: 10.1007/s00784-020-03452-9. Epub 2020 Jul 12.
It has been proposed that citrullination and carbamylation occur in the inflamed periodontium and could be the plausible mechanisms for the generation of antigens involved in the development and progression of RA. The purpose of this study was to determine the presence and location of citrullinated and carbamylated proteins in the gingival tissues and compare their abundance in periodontitis (PD) patients with or without RA.
Gingival tissue samples of healthy (n = 5), PD with RA (n = 5) and PD without RA (n = 5) were collected. Specimens were formalin fixed, paraffin embedded and sectioned at 4 μm. The tissue sections were analysed for the presence of citrullinated and carbamylated proteins by immunohistochemistry. Semi-quantitative analysis was performed to quantify and compare the protein abundance between groups.
The number of cells containing citrullinated and carbamylated proteins with higher intensity was markedly increased in gingival tissues from PD with or without RA in comparison with healthy controls.
Inflamed gingival tissue is a potential source of citrullinated and carbamylated proteins other than synovial tissues. The extent to which the local accumulation of these proteins contributes to the pathogenesis of RA needs further elucidation.
If PD is a potential source of post-translationally modified proteins, untreated PD should not be taken lightly in the context of RA. Hence, addressing gingival inflammation should be viewed as an important preventive measure in the general population not only for the progression of periodontal disease but also reducing the risk of developing extra-oral comorbidities.
有人提出，瓜氨酸化和氨甲酰化发生在炎症性牙周组织中，可能是产生与类风湿关节炎（RA）发展和进展相关抗原的合理机制。本研究的目的是确定瓜氨酸化和氨甲酰化蛋白在牙龈组织中的存在和位置，并比较 RA 伴或不伴 PD 患者中这些蛋白的丰度。
收集健康（n=5）、RA 伴 PD（n=5）和不伴 RA 的 PD（n=5）患者的牙龈组织样本。福尔马林固定、石蜡包埋并切成 4μm 厚的切片。通过免疫组织化学分析组织切片中瓜氨酸化和氨甲酰化蛋白的存在情况。进行半定量分析以量化和比较各组之间的蛋白丰度。
与健康对照组相比，RA 伴或不伴 PD 的牙龈组织中含有瓜氨酸化和氨甲酰化蛋白的细胞数量明显增加，且强度更高。
发炎的牙龈组织是除滑膜组织之外的瓜氨酸化和氨甲酰化蛋白的潜在来源。局部这些蛋白的积累对 RA 发病机制的影响程度需要进一步阐明。
如果 PD 是翻译后修饰蛋白的潜在来源，那么在 RA 背景下，未经治疗的 PD 不应被轻视。因此，在普通人群中，解决牙龈炎症不仅应被视为预防牙周病进展的重要措施，还应被视为降低发生口腔外合并症风险的重要措施。
