Lashak L K, Trebukhina R V, Ostrovskiĭ Iu M
Vopr Med Khim. 1978 Nov-Dec;24(6):822-6.
Administration of hydroxythiamin or thiamin into rats at a dose 400 mg/kg body weight caused a single-directed (unspecific, nonenzymatic) effect on the activity of glucose-6-phosphate and 6-phosphogluconate dehydrogenases within short periods (3-12 hrs). Within 24 and 72 hrs the effects of vitamin and antivitamin acquired the opposite (specific) direction towards the activity of glucose-6-phosphate dehydrogenase in liver tissues. Content of glucose-6-phosphate was altered depending on time of hydroxythiamin effect. Reverse correlation between the activity of glucose-6-phosphate dehydrogenase and content of the substrate was observed in heart muscle either after administration of hydroxythiamin or thiamin; the phenomenon suggest that dehydrogenase reactions affect considerably the regulation of glucose-6-phosphate content in the tissue.
以400毫克/千克体重的剂量给大鼠注射羟硫胺素或硫胺素，在短时间内（3 - 12小时）会对葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶的活性产生单向（非特异性、非酶促）影响。在24小时和72小时内，维生素和抗维生素对肝脏组织中葡萄糖-6-磷酸脱氢酶活性的影响呈现相反（特异性）方向。葡萄糖-6-磷酸的含量根据羟硫胺素作用时间而改变。在注射羟硫胺素或硫胺素后，心肌中葡萄糖-6-磷酸脱氢酶的活性与底物含量之间存在反向相关性；这一现象表明脱氢酶反应对组织中葡萄糖-6-磷酸含量的调节有相当大的影响。
