Dobos Attila, Kreizinger Zsuzsa, Kovács Áron B, Gyuranecz Miklós
CEVA-Phylaxia Zrt., Szállás u. 5., Budapest H-1107, Hungary.
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungária Körút 21, Budapest H-1143, Hungary.
Comp Immunol Microbiol Infect Dis. 2020 Oct;72:101489. doi: 10.1016/j.cimid.2020.101489. Epub 2020 May 13.
The aim of the present study was to assess the prevalence of Coxiella burnetii in dairy herds in Central and Eastern European countries based on ELISA and PCR tests. A total of 370 bulk tank milk samples were collected in 2019 originating from Croatia (n = 13), Czech Republic (n = 138), Hungary (n = 126), Serbia (n = 24), Slovakia (n = 53) and Slovenia (n = 16). Prevalence of C. burnetii differed according to the country of origin with Croatia showing 100.00%, the Czech Republic 98.55%, Hungary 97.61%, Serbia 70.83%, Slovakia 90.56% and Slovenia showing 62.50% average percentages of the positive herds. C. burnetii specific ELISA showed 100.00% positivity in all examined countries if herds consisted of equal or above 250 milking cows. The growing number of farms managing large number of animals, where cattle density is high correlates with the increasing prevalence of C. burnetii in the region.
本研究的目的是基于酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）测试，评估中东欧国家奶牛群中伯氏考克斯体的流行情况。2019年共收集了370份来自克罗地亚（n = 13）、捷克共和国（n = 138）、匈牙利（n = 126）、塞尔维亚（n = 24）、斯洛伐克（n = 53）和斯洛文尼亚（n = 16）的奶罐奶样本。伯氏考克斯体的流行率因原产国而异，克罗地亚为100.00%，捷克共和国为98.55%，匈牙利为97.61%，塞尔维亚为70.83%，斯洛伐克为90.56%，斯洛文尼亚阳性牛群的平均百分比为62.50%。如果牛群由250头或以上的奶牛组成，伯氏考克斯体特异性ELISA在所有被检查的国家都显示出100.00%的阳性率。管理大量动物且牛密度高的农场数量不断增加，这与该地区伯氏考克斯体流行率的上升相关。
