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用于纯化低聚葡萄籽原花青素的集成超滤/固相萃取工艺评估

Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins.

作者信息

Gutierrez-Docio Alba, Almodóvar Paula, Moreno-Fernandez Silvia, Silvan Jose Manuel, Martinez-Rodriguez Adolfo J, Alonso Gonzalo Luis, Prodanov Marin

机构信息

Functional Food Ingredients Group, Department of Production and Characterization of Novel Foods, Instituto de Investigación en Ciencias de la Alimentación (CIAL), (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain.

Pharmactive Biotech Products SL, Parque Científico de Madrid, 28049 Madrid, Spain.

出版信息

Membranes (Basel). 2020 Jul 9;10(7):147. doi: 10.3390/membranes10070147.

DOI:10.3390/membranes10070147
PMID:32660066
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7407673/
Abstract

The effectiveness of a preparative integrated ultrafiltration/solid-phase extraction (UF/SPE) process for purification of oligomeric procyanidins (OPCs) from a crude grape seed extract (GSE) was studied for the first time. The separation of OPCs from polymeric procyanidins (PPCs) by UF was very efficient. The membrane showed an acceptable filtration flux of 6 to 3.5 L/h·m at 0.5 bar of transmembrane pressure and 95% recovery of its water flux after chemical cleaning. The process was scalable to a pilot scale. The separation of very polar and ionic species from OPCs by SPE (XAD7HP and XAD16 resins) was also very good, but both adsorbents lost their retention capacities quickly, due probably to irreversible retention of OPCs/PPCs. Even though the global purification of OPCs by the integrated UF/SPE process allowed the recovery of 24.2 g of highly purified OPCs (83% purity) from 14.4 L of crude grape seed extract, the use of these adsorbents for further purification of the OPCs was very limited.

摘要

首次研究了制备性集成超滤/固相萃取（UF/SPE）工艺从粗葡萄籽提取物（GSE）中纯化低聚原花青素（OPC）的有效性。通过超滤从聚合原花青素（PPC）中分离OPC非常有效。在0.5巴的跨膜压力下，该膜显示出6至3.5升/小时·平方米的可接受过滤通量，化学清洗后其水通量回收率为95%。该工艺可扩大到中试规模。通过SPE（XAD7HP和XAD16树脂）从OPC中分离极性很强的和离子性物质也非常好，但两种吸附剂都很快失去了保留能力，这可能是由于OPC/PPC的不可逆保留。尽管通过集成UF/SPE工艺对OPC进行整体纯化能够从14.4升粗葡萄籽提取物中回收24.2克高纯度OPC（纯度83%），但使用这些吸附剂对OPC进行进一步纯化的效果非常有限。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/2ddda818d6cf/membranes-10-00147-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/ff9c71f2d525/membranes-10-00147-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/370d46421bee/membranes-10-00147-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/3866ab574096/membranes-10-00147-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/2939bf76947b/membranes-10-00147-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/94d3a4d633c0/membranes-10-00147-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/2ddda818d6cf/membranes-10-00147-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/ff9c71f2d525/membranes-10-00147-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/a4326d1418b4/membranes-10-00147-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/370d46421bee/membranes-10-00147-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/f639d3108146/membranes-10-00147-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/3866ab574096/membranes-10-00147-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/2939bf76947b/membranes-10-00147-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/94d3a4d633c0/membranes-10-00147-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171c/7407673/2ddda818d6cf/membranes-10-00147-g008.jpg

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