Qi Tao, Gao Hongxiang, Dang Yazheng, Huang Shigao, Peng Minfei
Department of Radiation Oncology, 986 Hospital of People's Liberation Army Air Force.
Department of Radiotherapy Oncology, Chang An Hospital, Xi'an, Shaan Xi.
Medicine (Baltimore). 2020 Jul 10;99(28):e21222. doi: 10.1097/MD.0000000000021222.
Cervus and cucumis peptides (Lugua polypeptides, LG) are traditional Chinese medicine, which are active components of polypeptide extracted from Sika deer bone and melon seed, and they contain bone induced polypeptide biological factors. Umbilical cord mesenchymal stem cell, (UC-MSC) have tissue repair multiple effects, anti-inflammatory, and immune regulation function, which become a very promising start in rheumatoid arthritis (RA) treatment. Hence, LG combined UC-MSC can significantly enhance the UC-MSC treatment of rheumatoid arthritis (RA).To explore the clinical curative effect and therapeutic mechanism of LG combined UC-MSC for treating RA.119 patients were divided into control and treatment groups, and both groups were treated with methotrexate tablets, leflunomide, and UC-MSC. But, LG were added to the treatment group. In vitro, the effects of LG on UC-MSC cell secretion of anti-inflammatory factors were also performed.The Health Assessment Questionnaire; the 28 joint disease activity score; C reactive protein; the erythrocyte sedimentation rate; rheumatoid factor; and anti-cyclic citrullinated peptide antibody were significantly reduced in treatment group 1 year after treatment (P < .05). In vitro, compared with the control group, the number of hepatocyte growth factor (HGF), the secretion of prostaglandin E2 (PGE2) and tumor necrosis factor-inducible gene 6 protein (TSG6) increased significantly (P < .05).LG combined UC-MSCs can significantly improve the curative effect of RA patients, while LG may reduce inflammatory cytokines, regulate immunity, improve microcirculation, and are conducive to UC-MSCs migration and the repair of damaged tissue.
鹿瓜多肽（Lugua polypeptides，LG）是中药，是从梅花鹿骨和瓜籽中提取的多肽活性成分，含有骨诱导多肽生物因子。脐带间充质干细胞（UC-MSC）具有组织修复、抗炎和免疫调节等多种作用，成为类风湿关节炎（RA）治疗中非常有前景的开端。因此，LG联合UC-MSC可显著增强UC-MSC对类风湿关节炎（RA）的治疗效果。为探讨LG联合UC-MSC治疗RA的临床疗效及作用机制。将119例患者分为对照组和治疗组，两组均采用甲氨蝶呤片、来氟米特及UC-MSC治疗，但治疗组加用LG。体外实验还观察了LG对UC-MSC细胞分泌抗炎因子的影响。治疗1年后，治疗组的健康评估问卷、28关节疾病活动评分、C反应蛋白、红细胞沉降率、类风湿因子及抗环瓜氨酸肽抗体均显著降低（P<0.05）。体外实验中，与对照组相比，治疗组肝细胞生长因子（HGF）数量、前列腺素E2（PGE2）及肿瘤坏死因子诱导基因6蛋白（TSG6）分泌显著增加（P<0.05）。LG联合UC-MSCs可显著提高RA患者的治疗效果，同时LG可能降低炎性细胞因子、调节免疫、改善微循环，有利于UC-MSCs迁移及受损组织修复。
