Sosa-Ochoa Wilfredo, Zúniga Concepción, Chaves Luis Fernando, Araujo Flores Gabriela Venicia, Sandoval Pacheco Carmen Maria, Ribeiro da Matta Vania Lúcia, Pereira Corbett Carlos Eduardo, Tobias Silveira Fernando, Dalastra Laurenti Marcia
Laboratory of Pathology of Infectious Diseases, Medical School, São Paulo University, São Paulo 01246-903, SP, Brazil.
Microbiology Research Institute, Universidad Nacional Autónoma de Honduras, Tegucigalpa 11101, Honduras.
Pathogens. 2020 Jul 10;9(7):554. doi: 10.3390/pathogens9070554.
is the etiological agent of both American visceral leishmaniasis (AVL) and non-ulcerated cutaneous leishmaniasis (NUCL) in Honduras. Although AVL is the most severe clinical form of infection, recent studies have shown that human immune response to parasite infection can result in a clinical-immunological spectrum. The overall prevalence rate of infection and clinical-immunological profiles of the infection in Amapala municipality, South Honduras was determined. We examined 576 individuals with diagnosis based on combined ELISA (IgG/IgM) and DTH assays. We also used genus-specific kDNA PCR and Hsp70 PCR-RFLP for NUCL cases. Clinical evaluation found 82% asymptomatic and 18% symptomatic individuals. All symptomatic cases (n = 104) showing NUCL were positive for parasites. We identified species in 100% of the skin lesion scrapings and in 90% of the blood samples from NUCL cases studied. A total of 320 asymptomatic individuals were exposed (ELISA+ and/or DTH+), providing an overall prevalence of 73.6%. Clinical, parasitological, and immunological evaluations suggest seven infection profiles, three asymptomatic and four symptomatic. This represents the first report on clinical and immunological features of human -infection in Amapala municipality, Honduras.
是洪都拉斯美洲内脏利什曼病（AVL）和非溃疡性皮肤利什曼病（NUCL）的病原体。尽管AVL是最严重的临床感染形式，但最近的研究表明，人类对寄生虫感染的免疫反应可导致临床-免疫谱。确定了洪都拉斯南部阿马帕拉市感染的总体患病率以及感染的临床-免疫特征。我们通过联合ELISA（IgG/IgM）和DTH检测对576名个体进行了诊断检查。对于NUCL病例，我们还使用了属特异性kDNA PCR和Hsp70 PCR-RFLP。临床评估发现82%为无症状个体，18%为有症状个体。所有表现为NUCL的有症状病例（n = 104）寄生虫检测均呈阳性。在我们研究的NUCL病例中，100%的皮肤病变刮片和90%的血液样本中鉴定出了 种。共有320名无症状个体暴露（ELISA+和/或DTH+），总体患病率为73.6%。临床、寄生虫学和免疫学评估显示有七种感染特征，三种无症状，四种有症状。这是关于洪都拉斯阿马帕拉市人类 感染的临床和免疫特征的首次报告。
