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微生物α-淀粉酶的生产：进展、挑战与展望

Microbial Alpha-Amylase Production: Progress, Challenges and Perspectives.

作者信息

Elyasi Far Babak, Ahmadi Yassin, Yari Khosroshahi Ahmad, Dilmaghani Azita

机构信息

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Adv Pharm Bull. 2020 Jul;10(3):350-358. doi: 10.34172/apb.2020.043. Epub 2020 May 11.

DOI:10.34172/apb.2020.043
PMID:32665893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7335993/
Abstract

Alpha-amylase reputes for starch modification by breaking of 1-4 glycosidic bands and is widely applied in different industrial sectors. Microorganisms express unique alpha-amylases with thermostable and halotolerant characteristics dependent on the microorganism's intrinsic features. Likewise, genetic engineering methods are applied to produce enzymes with higher stability in contrast to wild types. As there are widespread application of α-amylase in industry, optimization methods like RSM are used to improve the production of the enzyme ex vivo. This study aimed to review the latest researches on the production improvement and stability of α-amylase.

摘要

α-淀粉酶因能通过断裂1-4糖苷键来修饰淀粉而闻名，并且广泛应用于不同的工业领域。微生物会表达具有耐热和耐盐特性的独特α-淀粉酶，这些特性取决于微生物的内在特征。同样，与野生型相比，基因工程方法被用于生产具有更高稳定性的酶。由于α-淀粉酶在工业上有广泛应用，像响应面法这样的优化方法被用于提高该酶的体外产量。本研究旨在综述关于α-淀粉酶产量提高和稳定性的最新研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/87968a9f503a/apb-10-350-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/a86d3db2e593/apb-10-350-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/38dc7c9fe3f8/apb-10-350-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/9927380050b6/apb-10-350-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/71ba0f37d4df/apb-10-350-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/87968a9f503a/apb-10-350-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/a86d3db2e593/apb-10-350-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/38dc7c9fe3f8/apb-10-350-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/9927380050b6/apb-10-350-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/71ba0f37d4df/apb-10-350-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d0/7335993/87968a9f503a/apb-10-350-g005.jpg

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