Division of Microbiology, Department of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006401.
A polyphasic approach was used to characterize two novel actinobacterial strains, designated PKS22-38 and LSe1-13, which were isolated from mangrove soils and leaves of halophyte (L.), respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that they belonged to the genus and were most closely related to three validly published species with similarities ranging from 98.6 to 98.1 %. The genomic DNA G+C contents of strains PKS22-38 and LSe1-13 were 67.3 and 67.2 mol%, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains were 93.3 and 54.9 %, respectively, revealing that they are independent species. Meanwhile, the ANI and dDDH values between the two novel strains and closely related type strains were below 80.5 and 24.0 %, respectively. Strains PKS22-38 and LSe1-13 contained C, C 9 and C 10-methyl (TBSA) as the major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the main phospholipids. The predominant menaquinone was MK-9(H). Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strains PKS22-38 and LSe1-13 are considered to represent two novel species within the genus , for which the names sp. nov. and sp. nov. are proposed, with strain PKS22-38 (=TBRC 17540=NBRC 116256) and strain LSe1-13 (=TBRC 17706=NBRC 116396) as the type strains, respectively.
采用多相分类法对两株新型放线菌进行了研究，分别命名为 PKS22-38 和 LSe1-13，它们分别从红树林土壤和盐生植物 (L.)叶片中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，它们属于 属，与三个有效发表的种最为密切相关，相似度为 98.6%至 98.1%。菌株 PKS22-38 和 LSe1-13 的基因组 DNA G+C 含量分别为 67.3mol%和 67.2mol%。两株菌之间的平均核苷酸同一性 (ANI) 和数字 DNA-DNA 杂交 (dDDH) 值分别为 93.3%和 54.9%，表明它们是独立的种。同时，两株新菌株与亲缘关系密切的模式菌株之间的 ANI 和 dDDH 值分别低于 80.5%和 24.0%。菌株 PKS22-38 和 LSe1-13 含有 C 、 C9 和 C10-甲基 (TBSA) 作为主要脂肪酸和双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇作为主要磷脂。主要的menaquinone 是 MK-9(H)。基于表型、化学分类、系统发育和基因组数据，菌株 PKS22-38 和 LSe1-13 被认为代表 属中的两个新种，分别命名为 sp. nov. 和 sp. nov.，其中菌株 PKS22-38(=TBRC 17540=NBRC 116256)和菌株 LSe1-13(=TBRC 17706=NBRC 116396)分别为模式菌株。
