Graduate School of Science and Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
J Biosci Bioeng. 2020 Oct;130(4):402-408. doi: 10.1016/j.jbiosc.2020.06.004. Epub 2020 Jul 12.
Aerobic fed-batch cultures were studied as a means of suppressing the production of lactate, which inhibits the growth of lactic acid bacteria (LAB). LAB produce lactate via lactate dehydrogenase (LDH), regenerating nicotinamide adenine dinucleotide (NAD) consumed during glycolysis. Therefore, we focused on NADH oxidase (NOX), employing oxygen as an electron acceptor, as an alternative pathway to LDH for NAD regeneration. To avoid glucose repression of NOX and NAD consumption by glycolysis exceeding NAD regeneration by NOX, glucose was fed gradually. When Lactococcus lactis MG 1363 was aerobically fed at a specific growth rate of 0.2 h, the amount of lactate produced per amount of grown cell was reduced to 12% of that in anaerobic batch cultures. Metabolic flux analysis revealed that in addition to NAD regeneration by NOX, ATP acquisition by production of acetate and NAD regeneration by production of acetoin and 2,3-butanediol contributed to suppression of lactate production.
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