Cran D G, Moor R M, Irvine R F
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
J Cell Sci. 1988 Sep;91 ( Pt 1):139-44. doi: 10.1242/jcs.91.1.139.
Microinjection of inositol 1,4,5-triphosphate into sheep and hamster oocytes induces secretion of cortical granules in a dose-dependent manner. In the sheep, this effect is strongly pH-dependent with minimal exocytosis taking place at pH 6.8 but a full cortical reaction occurring at pH8.0. Exocytosis in the hamster is also affected by the pH of the external medium but to a lesser extent. Injection of GTP gamma S also induces exocytosis in both species but is more effective in the hamster. It is suggested that inositol metabolism stimulated by sperm-egg interaction with a GTP-binding protein may be part of the mechanism leading to cortical granule exocytosis and that this may be modulated by the external pH.
将肌醇1,4,5 - 三磷酸显微注射到绵羊和仓鼠卵母细胞中，会以剂量依赖的方式诱导皮质颗粒的分泌。在绵羊中，这种效应强烈依赖于pH值，在pH 6.8时发生最小程度的胞吐作用，而在pH 8.0时发生完全的皮质反应。仓鼠中的胞吐作用也受外部介质pH值的影响，但程度较小。注射GTPγS也会在这两个物种中诱导胞吐作用，但在仓鼠中更有效。有人提出，精子与卵相互作用通过GTP结合蛋白刺激的肌醇代谢可能是导致皮质颗粒胞吐作用机制的一部分，并且这可能受外部pH值的调节。
