Mozafarizadeh Mina, Parvizi Omran Sima, Kordestani Zeinab, Manshadi Dehghan Hamidreza, Faridazar Alireza, Houshmand Massoud
Department of Cellular and Molecular Biology, Nour Danesh Institute of Higher Education, Isfahan, Iran.
Houshmand Genetic Diagnostics Laboratory, Taban Clinic, Tehran, Iran.
Iran J Biotechnol. 2019 Dec 1;17(4):e2460. doi: 10.30498/IJB.2019.99594. eCollection 2019 Dec.
Heterogeneous breast cancer is the most common cause of cancer-related mortality. Obesity defined by BMI is a known major risk factor for breast cancer.
The purpose of this study was to explore the role of obesity related-polymorphisms rs9939609 Fat Mass and Obesity-associated (FTO) and rs17782313 in breast cancer development.
Matched peripheral blood serum was obtained from 64 breast cancer patients and 83 normal controls. Height and weight were measured to calculate BMI. All were genotyped for the SNPs rs9939609 and rs17782313 using a Tetra-primer ARMS-PCR method. For statistical analysis, the chi-square test and SPSS software were used.
In subgroup analyses defined by BMI, rs9939609 genotypes (TT/AA/AT) were significantly associated with the risk of breast cancer only in non-obese subjects ( < 0.005). TT genotypes of rs17782313 in non-obese and genotypes TT/CC in the overweight group were also statistically associated with breast cancer ( < 0.005). No significant associations between any variants and breast cancer risk were seen in obese subjects.
Based on the absence of an association between obesity-related SNPs and breast cancer in obese subjects, it is proposed that weight gain in Iranian women will help prevent breast cancer risk. The result help for preparing and designing a safe and versatile recombinant drug in future.
异质性乳腺癌是癌症相关死亡的最常见原因。由体重指数（BMI）定义的肥胖是已知的乳腺癌主要危险因素。
本研究的目的是探讨肥胖相关多态性rs9939609脂肪量与肥胖相关基因（FTO）和rs17782313在乳腺癌发生中的作用。
从64例乳腺癌患者和83例正常对照中获取配对的外周血血清。测量身高和体重以计算BMI。使用四引物扩增受阻突变系统聚合酶链反应（Tetra-primer ARMS-PCR）方法对所有研究对象进行rs9939609和rs17782313单核苷酸多态性（SNP）基因分型。采用卡方检验和SPSS软件进行统计分析。
在按BMI定义的亚组分析中，rs993960基因型（TT/AA/AT）仅在非肥胖受试者中与乳腺癌风险显著相关（<0.005）。rs17782313的TT基因型在非肥胖组以及超重组中的CC基因型也与乳腺癌存在统计学关联（<0.005）。在肥胖受试者中未发现任何变异与乳腺癌风险之间存在显著关联。
基于肥胖受试者中肥胖相关SNP与乳腺癌之间不存在关联，有人提出伊朗女性体重增加将有助于预防乳腺癌风险。该结果有助于未来制备和设计一种安全且通用的重组药物。
