Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599.
ACS Biomater Sci Eng. 2020 Jan 13;6(1):433-441. doi: 10.1021/acsbiomaterials.9b01384. Epub 2019 Nov 13.
Nitric oxide (NO) is a broad-spectrum antibacterial agent, making it an attractive alternative to traditional antibiotics for treating infections. To date, a direct comparison of the antibacterial activity of gaseous NO (gNO) versus water-soluble NO-releasing biopolymers has not been reported. In this study, the bactericidal action of NO-releasing chitosan oligosaccharides was compared to gNO treatment against cystic fibrosis-relevant Gram-positive and Gram-negative bacteria. A NO exposure chamber was constructed to enable the dosing of bacteria with gNO at concentrations up to 800 ppm under both aerobic and anaerobic conditions. Bacteria viability, solution properties (i.e., pH, NO concentration), and toxicity to mammalian cells were monitored to ensure a thorough understanding of bactericidal action and reproducibility for each delivery method. The NO-releasing chitosan oligosaccharides required significantly lower NO doses relative to gNO therapy to elicit antibacterial action against and under both aerobic and anaerobic conditions. Reduced NO doses required for bacteria eradication using water-soluble NO-releasing chitosan were attributed to the release of NO in solution, removing the need to transfer from gas to liquid phase and the associated long diffusion distances of gNO treatment.
一氧化氮（NO）是一种广谱抗菌剂，作为传统抗生素的替代品，用于治疗感染具有很大吸引力。迄今为止，还没有报道过气态一氧化氮（gNO）与水溶性释放一氧化氮的生物聚合物的抗菌活性的直接比较。在这项研究中，比较了释放一氧化氮的壳聚糖低聚糖对囊性纤维化相关革兰氏阳性和革兰氏阴性细菌的杀菌作用与 gNO 处理的效果。构建了一个一氧化氮暴露室，使细菌在有氧和厌氧条件下能够以高达 800 ppm 的浓度接受 gNO 处理。监测了细菌的存活能力、溶液特性（即 pH 值、NO 浓度）以及对哺乳动物细胞的毒性，以确保对每种输送方法的杀菌作用和重现性有一个全面的了解。与 gNO 治疗相比，释放一氧化氮的壳聚糖低聚糖在有氧和厌氧条件下对 和 发挥抗菌作用所需的 NO 剂量要低得多。使用水溶性释放一氧化氮的壳聚糖可以减少杀灭细菌所需的 NO 剂量，这归因于 NO 在溶液中的释放，无需从气相转移到液相，以及 gNO 治疗的相关长扩散距离。
