有氧和无氧条件下一氧化氮释放壳寡糖对铜绿假单胞菌的抗菌作用
Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions.
作者信息
Reighard Katelyn P, Schoenfisch Mark H
机构信息
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
出版信息
Antimicrob Agents Chemother. 2015 Oct;59(10):6506-13. doi: 10.1128/AAC.01208-15. Epub 2015 Aug 3.
Abstract
Chitosan oligosaccharides were modified with N-diazeniumdiolates to yield biocompatible nitric oxide (NO) donor scaffolds. The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial activities were primarily the result of NO scavenging by oxygen under aerobic environments and not changes in bacterial physiology. Bacterial killing was also tested against nonmucoid and mucoid biofilms and compared to that of tobramycin. Smaller NO payloads were required to eradicate P. aeruginosa biofilms under anaerobic versus aerobic conditions. Under oxygen-free environments, the NO treatment was 10-fold more effective at killing biofilms than tobramycin. These results demonstrate the potential utility of NO-releasing chitosan oligosaccharides under both aerobic and anaerobic environments.
摘要
壳寡糖用二氮烯二醇盐进行修饰，以产生具有生物相容性的一氧化氮（NO）供体支架。在需氧和厌氧条件下，比较了NO供体对铜绿假单胞菌的最低杀菌浓度和最低抑菌浓度。不同的抗菌活性主要是由于在有氧环境中氧气对NO的清除作用，而非细菌生理状态的改变。还测试了对非黏液性和黏液性生物膜的杀菌效果，并与妥布霉素进行比较。在厌氧条件下与需氧条件相比，根除铜绿假单胞菌生物膜所需的NO负载量更小。在无氧环境下，NO处理杀灭生物膜的效果比妥布霉素高10倍。这些结果证明了在有氧和厌氧环境下释放NO的壳寡糖的潜在用途。
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