使用释放一氧化氮的壳寡糖破坏和根除铜绿假单胞菌生物膜
Disruption and eradication of P. aeruginosa biofilms using nitric oxide-releasing chitosan oligosaccharides.
作者信息
Reighard Katelyn P, Hill David B, Dixon Graham A, Worley Brittany V, Schoenfisch Mark H
机构信息
a Department of Chemistry , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
b The Marsico Lung Institute , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
出版信息
Biofouling. 2015;31(9-10):775-87. doi: 10.1080/08927014.2015.1107548.
Biofilm disruption and eradication were investigated as a function of nitric oxide- (NO) releasing chitosan oligosaccharide dose and the results compared with control (i.e., non-NO-releasing) chitosan oligosaccharides and tobramycin. Quantification of biofilm expansion/contraction and multiple-particle tracking microrheology were used to assess the structural integrity of the biofilm before and after antibacterial treatment. While tobramycin had no effect on the physical properties of the biofilm, NO-releasing chitosan oligosaccharides exhibited dose-dependent behavior with biofilm degradation. Control chitosan oligosaccharides increased biofilm elasticity, indicating that the scaffold may mitigate the biofilm disrupting power of nitric oxide somewhat. The results from this study indicate that nitric oxide-releasing chitosan oligosaccharides act as dual-action therapeutics capable of eradicating and physically disrupting P. aeruginosa biofilms.
研究了释放一氧化氮（NO）的壳寡糖剂量对生物膜破坏和根除的作用，并将结果与对照（即不释放NO的）壳寡糖和妥布霉素进行比较。通过生物膜扩张/收缩的定量分析和多粒子跟踪微流变学来评估抗菌处理前后生物膜的结构完整性。虽然妥布霉素对生物膜的物理性质没有影响，但释放NO的壳寡糖表现出与生物膜降解相关的剂量依赖性行为。对照壳寡糖增加了生物膜的弹性，表明该支架可能在一定程度上减轻一氧化氮对生物膜的破坏作用。本研究结果表明，释放一氧化氮的壳寡糖作为双作用疗法，能够根除并物理破坏铜绿假单胞菌生物膜。
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