Department of Urology, Zigong First People's Hospital, Zigong, Sichuan, China.
Department of Basic Medicine, Sichuan Vocational College of Health and Rehabilitation, Zigong, Sichuan, China.
Cell Biol Int. 2020 Oct;44(10):2131-2139. doi: 10.1002/cbin.11421. Epub 2020 Jul 28.
Multiple studies have confirmed the pro-oncogenic effects of PAX3 in an array of cancers, but its role in prostate cancer (PCa) remains largely undefined. The aim of this study is to investigate the role of PAX3 in PCa. PAX3 expression was compared between PCa tumor tissue and nontumor tissues and PCa cell lines and normal prostate epithelial cells (PNT2) by western blot analysis and immunohistochemistry staining. MTT and immunofluorescence assays were used to detect PCa cell proliferation. Flow cytometry was used to evaluate cell apoptosis in PCa. Transwell assays were used for the determination of cell migration and PCa cell invasion. PAX3 expression was higher in PCa tissues and human PCa cell lines. Moreover, PAX3 silencing inhibited the proliferation, metastasis, and epithelial-mesenchymal transition (EMT) of PCa cells, and increased the rates of apoptosis. PAX3 silencing inhibited transforming growth factor-β (TGF-β)/Smad signaling in PCa cells. The effects of si-PAX3 on the proliferation, apoptosis, metastasis, and EMT of PCa cells were alleviated by TGF-β1 treatment. PAX3 silencing inhibits PCa progression through the inhibition of TGF-β/Smad signaling. This reveals PAX3 as a novel biomarker and therapeutic target for future PCa treatments.
多项研究证实 PAX3 在多种癌症中具有致癌作用，但它在前列腺癌（PCa）中的作用在很大程度上仍未确定。本研究旨在探讨 PAX3 在 PCa 中的作用。通过 Western blot 分析和免疫组化染色比较了 PCa 肿瘤组织与非肿瘤组织以及 PCa 细胞系与正常前列腺上皮细胞（PNT2）之间的 PAX3 表达。MTT 和免疫荧光测定用于检测 PCa 细胞增殖。流式细胞术用于评估 PCa 细胞凋亡。Transwell 测定用于测定细胞迁移和 PCa 细胞侵袭。PAX3 在 PCa 组织和人 PCa 细胞系中表达较高。此外，PAX3 沉默抑制了 PCa 细胞的增殖、转移和上皮-间充质转化（EMT），并增加了细胞凋亡率。PAX3 沉默抑制了 PCa 细胞中的转化生长因子-β（TGF-β）/Smad 信号通路。TGF-β1 处理减轻了 si-PAX3 对 PCa 细胞增殖、凋亡、转移和 EMT 的影响。PAX3 沉默通过抑制 TGF-β/Smad 信号通路抑制 PCa 进展。这揭示了 PAX3 作为未来 PCa 治疗的新型生物标志物和治疗靶点。
