Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA; Department of Bioengineering, University of Washington, Seattle, WA, USA.
Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA; Department of Pathology, University of Washington, Seattle, WA, USA.
Trends Biotechnol. 2020 Aug;38(8):835-845. doi: 10.1016/j.tibtech.2020.01.006. Epub 2020 Mar 5.
Recent advances in stem cell biology and tissue engineering have laid the groundwork for building complex tissues in a dish. We propose that these technologies are ready for a new challenge: recapitulating cardiac morphogenesis in vitro. In development, the heart transforms from a simple linear tube to a four-chambered organ through a complex process called looping. Here, we re-examine heart tube looping through the lens of an engineer and argue that the linear heart tube is an advantageous starting point for tissue engineering. We summarize the structures, signaling pathways, and stresses in the looping heart, and evaluate approaches that could be used to build a linear heart tube and guide it through the process of looping.
近年来，干细胞生物学和组织工程学的进展为在培养皿中构建复杂组织奠定了基础。我们提出，这些技术已经准备好迎接新的挑战：在体外再现心脏形态发生。在发育过程中，心脏通过一个称为环化的复杂过程从一个简单的线性管转变为四个腔室的器官。在这里，我们从工程师的角度重新审视心脏管环化，并认为线性心脏管是组织工程的一个有利起点。我们总结了环化心脏中的结构、信号通路和应力，并评估了可用于构建线性心脏管并引导其环化的方法。
