载有乌头酸的脂质体明胶膜：角膜愈合的新治疗策略。
Gelatin-based membrane containing usnic acid-loaded liposomes: A new treatment strategy for corneal healing.
机构信息
Programa de Pós graduação em Ciências Aplicadas à Saúde, Universidade Federal de Sergipe, SE, 49400-000, Brazil; Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
出版信息
Biomed Pharmacother. 2020 Oct;130:110391. doi: 10.1016/j.biopha.2020.110391. Epub 2020 Jul 13.
PURPOSE
To evaluate the safety and potential healing efficacy of the topical ocular administration of a gelatin membrane containing usnic acid/liposomes (UALs) for corneal cicatrization. UALs have shown healing activity in animal models of dermal burn lesions. We evaluated the safety of topical ocular administration of UAL and its potential healing efficacy as an ophthalmic treatment on chemical lesions in rabbit eyes.
METHOD
The Draize test was used to check for ocular toxicity and the score was zero at each observation, indicating the ocular safety of a gelatin membrane containing usnic acid/liposome. Its potential healing efficacy as an ophthalmic treatment on chemical lesions in rabbit eyes was also assessed.
RESULTS
After epithelial removal and treatment with UAL, there was a 49.4 % reduction in injury under in vivo conditions compared with a 36.6 % reduction in the control, a gelatin membrane containing liposome without usnic acid. Histological analysis of ocular surface chemical injury-tissue sections after treatment with UAL supported these observations. The corneal expression of VEGF and TGF-β1increased by 70 % and 50 % respectively following treatment with UAL gelatin membrane.
CONCLUSION
These results indicate the potential therapeutic application of UAL gelatin membranes as an ophthalmic treatment that may be used for corneal cicatrization.
目的
评估载有乌头酸/脂质体（UAL）的明胶膜局部眼内给药的安全性和潜在的治疗效果，用于治疗角膜瘢痕。UAL 在皮肤烧伤动物模型中表现出了治疗活性。我们评估了局部眼内给予 UAL 的安全性及其作为眼部治疗药物对兔眼化学损伤的潜在治疗效果。
方法
采用 Draize 试验检查眼毒性，每次观察的评分均为零，表明载有乌头酸/脂质体的明胶膜具有眼部安全性。还评估了其作为眼部治疗药物对兔眼化学损伤的潜在治疗效果。
结果
与不含乌头酸的脂质体明胶膜对照组相比，在体内条件下，上皮去除和 UAL 治疗后损伤减少了 49.4%，而对照组为 36.6%。经 UAL 处理后的眼表面化学损伤组织切片的组织学分析支持了这些观察结果。角膜中 VEGF 和 TGF-β1 的表达分别增加了 70%和 50%。
结论
这些结果表明，UAL 明胶膜作为一种可能用于治疗角膜瘢痕的眼部治疗药物具有潜在的治疗应用。
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