Nunes Paula Santos, Rabelo Alessandra Silva, Souza Jamille Cristina Campos de, Santana Bruno Vasconcelos, da Silva Thailson Monteiro Menezes, Serafini Mairim Russo, Dos Passos Menezes Paula, Dos Santos Lima Bruno, Cardoso Juliana Cordeiro, Alves Júlio César Santana, Frank Luiza Abrahão, Guterres Sílvia Stanisçuaski, Pohlmann Adriana Raffin, Pinheiro Malone Santos, de Albuquerque Ricardo Luiz Cavalcanti, Araújo Adriano Antunes de Souza
Department of Morphology, Federal University of Sergipe, Marechal Rondom Avenue s/n, São Cristóvão, SE, Brazil.
Department of Pharmacy, Federal University of Sergipe, Marechal Rondom Avenue s/n, São Cristóvão, SE, Brazil.
Int J Pharm. 2016 Nov 20;513(1-2):473-482. doi: 10.1016/j.ijpharm.2016.09.040. Epub 2016 Sep 12.
There are a range of products available which claim to accelerate the healing of burns; these include topical agents, interactive dressings and biomembranes. The aim of this study was to assess the effect of a gelatin-based membrane containing usnic acid/liposomes on the healing of burns in comparison to silver sulfadiazine ointment and duoDerme dressing, as well as examining its quantification by high performance liquid chromatography. The quantification of the usnic acid/liposomes was examined using high performance liquid chromatography (HPLC) by performing separate in vitro studies of the efficiency of the biomembranes in terms of encapsulation, drug release and transdermal absorption. Then, second-degree 5cm burn wounds were created on the dorsum of nine male pigs, assigned into three groups (n=3): SDZ - animals treated with silver sulfadiazine ointment; GDU - animals treated with duoDerme; UAL - animals treated with a gelatin-based membrane containing usnic acid/liposomes. These groups were treated for 8, 18 and 30days. In the average rate of contraction, there was no difference among the groups (p>0.05). The results of the quantification showed that biomembranes containing usnic acid/liposomes were controlled released systems capable of transdermal absorption by skin layers. A macroscopic assay did not observe any clinical signs of secondary infections. Microscopy after 8days showed hydropic degeneration of the epithelium, with intense neutrophilic infiltration in all three groups. At 18days, although epidermal neo-formation was only partial in all three groups, it was most incipient in the SDZ group. Granulation tissue was more exuberant and cellularized in the UAL and GDU groups. At 30days, observed restricted granulation tissue in the region below the epithelium in the GDU and UAL groups was observed. In the analysis of collagen though picrosirius, the UAL group showed greater collagen density. Therefore, the UAL group displayed development and maturation of granulation tissue and scar repair that was comparable to that produced by duoDerme and better than that produced by treatment with sulfadiazine silver ointment In addition, the UAL group showed increased collagen deposition compared to the other two groups.
有一系列声称能加速烧伤愈合的产品；这些产品包括局部用药、交互式敷料和生物膜。本研究的目的是评估含松萝酸/脂质体的明胶基膜与磺胺嘧啶银软膏和多爱肤敷料相比对烧伤愈合的影响，并通过高效液相色谱法对其进行定量分析。使用高效液相色谱法（HPLC）通过对生物膜在包封、药物释放和透皮吸收方面的效率进行单独的体外研究来检测松萝酸/脂质体的定量。然后，在九只雄性猪的背部创建5厘米的二度烧伤创面，分为三组（n = 3）：SDZ组——用磺胺嘧啶银软膏治疗的动物；GDU组——用多爱肤治疗的动物；UAL组——用含松萝酸/脂质体的明胶基膜治疗的动物。这些组分别治疗8天、18天和30天。在平均收缩率方面，各组之间没有差异（p>0.05）。定量分析结果表明，含松萝酸/脂质体的生物膜是能够被皮肤层透皮吸收的控释系统。宏观分析未观察到任何继发感染的临床症状。8天后的显微镜检查显示上皮细胞出现水样变性，所有三组均有强烈的中性粒细胞浸润。18天时，尽管所有三组的表皮新生均仅为部分，但在SDZ组中最为初期。UAL组和GDU组的肉芽组织更旺盛且细胞化。30天时，在GDU组和UAL组的上皮下方区域观察到有限的肉芽组织。通过天狼星苦味酸染色分析胶原蛋白时，UAL组显示出更高的胶原蛋白密度。因此，UAL组显示出肉芽组织的发育和成熟以及瘢痕修复，与多爱肤相当且优于磺胺嘧啶银软膏治疗。此外，与其他两组相比，UAL组的胶原蛋白沉积增加。
