CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 88 Xuefu Road, Kunming 650223, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2020 Jul 14;21(14):4968. doi: 10.3390/ijms21144968.
Plant height is a vital agronomic trait that greatly determines crop yields because of the close relationship between plant height and lodging resistance. Legumes play a unique role in the worldwide agriculture; however, little attention has been given to the molecular basis of their height. Here, we characterized the first dwarf mutant () of the model legume plant . Our study found that both cell length and the cell number of internodes were reduced in a mutant. Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned the gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. MNP1 was highly homologous to LS. The subcellular localization showed that MNP1 was located in the chloroplast. Further analysis indicated that GA could significantly restore the plant height of , and expression of in a mutant of partially rescued its mini-plant phenotype, indicating the conservation function of MNP1 in GA biosynthesis. Our results provide valuable information for understanding the genetic regulation of plant height in
株高是一个重要的农艺性状，因为它与倒伏抗性密切相关，所以极大地决定了作物的产量。豆科植物在全球农业中起着独特的作用；然而，它们的高度的分子基础却很少受到关注。在这里，我们对模式豆科植物 的第一个矮秆突变体 () 进行了表征。我们的研究发现，突变体中节间的细胞长度和细胞数量都减少了。通过正向遗传筛选和随后的全基因组重测序方法，我们克隆了 基因，并发现它编码一个假定的焦磷酸合酶（CPS），该酶参与赤霉素（GA）生物合成的第一步。MNP1 与 LS 高度同源。亚细胞定位表明 MNP1 位于叶绿体中。进一步的分析表明，GA 可以显著恢复 的株高，并且在 的突变体中表达 部分挽救了其迷你植物表型，表明 MNP1 在 GA 生物合成中的保守功能。我们的研究结果为理解豆科植物株高的遗传调控提供了有价值的信息。
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