不结球白菜（亚种）中冷休克蛋白3（BcCSP3）的鉴定与表达分析

Identification and Expression Analysis of Cold Shock Protein 3 (BcCSP3) in Non-Heading Chinese Cabbage ( ssp. ).

作者信息

Huang Feiyi, Wang Jin, Duan Weike, Hou Xilin

机构信息

State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture/Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences and Food Engineering, Huaiyin Institute of Technology, Huai an 223003, China.

出版信息

Plants (Basel). 2020 Jul 14;9(7):890. doi: 10.3390/plants9070890.

DOI:10.3390/plants9070890

PMID:32674472

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7412364/

Abstract

A cold-related protein, cold shock protein 3 (BcCSP3), was isolated from non-heading Chinese cabbage in this study. BcCSP3 can encode 205 amino acids (aa) with an open reading frame (ORF) of 618 base pairs (bp). Multiple sequence alignment and phylogenetic tree analyses showed that BcCSP3 contains an N-terminal cold shock domain and is highly similar to AtCSP2, their kinship is recent. Real-time quantitative polymerase chain reaction (RT-qPCR) showed that the expression level of in stems and leaves is higher than that in roots. Compared with other stress treatments, the change in expression level was most pronounced under cold stress. In addition, a fusion protein was localized to the nucleus and cytoplasm. These results indicated that BcCSP3 may play an important role in response to cold stress in non-heading Chinese cabbage. This work may provide a reference for the identification and expression analysis of other genes in non-heading Chinese cabbage.

摘要

本研究从不结球白菜中分离出一种与低温相关的蛋白——冷休克蛋白3（BcCSP3）。BcCSP3可编码205个氨基酸，其开放阅读框（ORF）为618个碱基对（bp）。多重序列比对和系统发育树分析表明，BcCSP3含有一个N端冷休克结构域，与AtCSP2高度相似，它们的亲缘关系较近。实时定量聚合酶链反应（RT-qPCR）表明，其在茎和叶中的表达水平高于根中。与其他胁迫处理相比，低温胁迫下其表达水平变化最为明显。此外，一种融合蛋白定位于细胞核和细胞质中。这些结果表明，BcCSP3可能在不结球白菜对低温胁迫的响应中起重要作用。这项工作可为不结球白菜中其他相关基因的鉴定和表达分析提供参考。