From the Department of Neurosurgery (S.Q., T.H., O.Q., Y.S., Z.X.), First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Department of Neurosurgery (J.G.), Cancer Center, Sun Yat-sen University, Guangzhou, China.
AJNR Am J Neuroradiol. 2020 Aug;41(8):1423-1429. doi: 10.3174/ajnr.A6638. Epub 2020 Jul 16.
Previous studies have suggested that increased mortality and disability in patients with brain tumor are associated with peritumoral brain edema. However, the mechanism of peritumoral brain edema in brain tumors is unknown. This study aimed to investigate the effect of Piezo1 overexpression on peritumoral brain edema in glioblastomas.
The Piezo1 expression in cell lines and paired samples was detected by quantitative reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry. Sixty-four patients with glioblastomas were analyzed retrospectively. The Piezo1 expression of tumor tissue was detected by immunohistochemistry. The diameters of tumor and edema were measured by preoperative MR imaging, and the edema index value was calculated.
Western blot and quantitative reverse transcription polymerase chain reaction showed that Piezo1 expression was higher in 6 glioma cell lines than in the normal astrocyte cell line. Compared with peritumoral tissues, Piezo1 was up-regulated in tumor tissues. Sixty-four patients with glioblastomas were enrolled in further study. Piezo1 was higher in the moderate edema group than in the mild edema group ( < .001), higher in the severe edema group than in the moderate edema group ( < .001), and correlated with the edema index ( = 0.73; < .001). Receiver operating characteristic curve analysis showed that the edema index yielded an area under the curve of 0.867 (95% CI, 0.76-0.97; < .001), with a sensitivity of 100% and a specificity of 70%.
Piezo1 overexpression is positively correlated with the degree of peritumoral brain edema in glioblastomas. Predicting high Piezo1 expression in tumor tissues based on the edema extent shows good sensitivity and specificity.
先前的研究表明，脑肿瘤患者的死亡率和残疾率升高与肿瘤周围脑水肿有关。然而，脑肿瘤周围脑水肿的机制尚不清楚。本研究旨在探讨 Piezo1 过表达对脑胶质母细胞瘤周围脑水肿的影响。
通过定量逆转录聚合酶链反应、Western blot 和免疫组织化学检测细胞系和配对样本中的 Piezo1 表达。回顾性分析 64 例脑胶质母细胞瘤患者。通过免疫组织化学检测肿瘤组织中 Piezo1 的表达。术前磁共振成像测量肿瘤和水肿的直径，并计算水肿指数值。
Western blot 和定量逆转录聚合酶链反应显示，6 种神经胶质瘤细胞系中的 Piezo1 表达高于正常星形胶质细胞系。与肿瘤周围组织相比，Piezo1 在肿瘤组织中上调。进一步纳入 64 例脑胶质母细胞瘤患者进行研究。中度水肿组 Piezo1 高于轻度水肿组（<0.001），重度水肿组高于中度水肿组（<0.001），与水肿指数呈正相关（=0.73；<0.001）。受试者工作特征曲线分析显示，水肿指数的曲线下面积为 0.867（95%CI，0.76-0.97；<0.001），敏感性为 100%，特异性为 70%。
Piezo1 过表达与脑胶质母细胞瘤周围脑水肿的程度呈正相关。根据水肿程度预测肿瘤组织中 Piezo1 的高表达具有良好的敏感性和特异性。
