Jeon Se Yeong, Kim Mi Ran, Yu Su Hyun, Kim Min Jung, Shim Kyu-Suk, Shin Eunju, Lee Jeong Jun, Lee Young Chul
R&D Center, Naturetech Co., Ltd., Chungnam 31257, Korea.
Univera Co., Ltd., Seoul 04782, Korea.
Prev Nutr Food Sci. 2020 Jun 30;25(2):173-183. doi: 10.3746/pnf.2020.25.2.173.
Endothelial cell injury caused by oxidative stress is a critical factor in the initial stage of vascular diseases. Thus, identification of more effective antioxidants is a promising strategy to protect against endothelial cell injury. Recently, synergistic effects between phytochemicals have received renewed attention for their role in the treatment of various diseases. L. and are well-known medicinal plants with various biological effects. However, the combination of the two has not previously been studied. Here, we investigated the effects of L. leaf and extract combination (VCEC), a standardized herbal blend comprising L. leaf extract (VE) and extract (CE), for its antioxidant activity and for the protection of endothelial cells against hydrogen peroxide (HO)-mediated oxidative damage in human umbilical vein endothelial cells (HUVECs). VCEC showed higher antioxidant activity than VE or CE in oxygen radical antioxidant capacity assays. In HUVECs, VCEC significantly suppressed increases in the production of intracellular reactive oxygen species, decreased levels of nitric oxide and vascular endothelial-cadherin, and increased endothelial hyperpermeability triggered by HO. Treatment with VE or CE alone ameliorated HUVEC injury in a pattern similar to VCEC, although their effects were significantly weaker than VCEC. Overall, VCEC exhibited a substantial synergistic effect on protecting endothelial cells against oxidative damage through its antioxidant activity. Therefore, VCEC could be developed as a potential agent for reducing the risk of vascular diseases related to oxidative stress.
氧化应激引起的内皮细胞损伤是血管疾病初始阶段的关键因素。因此，鉴定更有效的抗氧化剂是预防内皮细胞损伤的一种有前景的策略。最近，植物化学物质之间的协同作用因其在各种疾病治疗中的作用而重新受到关注。L.和是具有多种生物学效应的著名药用植物。然而，此前尚未对两者的组合进行研究。在此，我们研究了L.叶和提取物组合（VCEC）的作用，VCEC是一种标准化草药混合物，由L.叶提取物（VE）和提取物（CE）组成，研究其抗氧化活性以及对人脐静脉内皮细胞（HUVECs）中过氧化氢（HO）介导的氧化损伤的内皮细胞保护作用。在氧自由基抗氧化能力测定中，VCEC显示出比VE或CE更高的抗氧化活性。在HUVECs中，VCEC显著抑制细胞内活性氧生成的增加，降低一氧化氮和血管内皮钙黏蛋白水平，并减轻HO引发的内皮细胞高通透性。单独用VE或CE处理改善HUVEC损伤的模式与VCEC相似，尽管它们的作用明显弱于VCEC。总体而言，VCEC通过其抗氧化活性在保护内皮细胞免受氧化损伤方面表现出显著的协同作用。因此，VCEC可被开发为一种潜在药物，以降低与氧化应激相关的血管疾病风险。
