Sangiovanni Enrico, Di Lorenzo Chiara, Piazza Stefano, Manzoni Yuri, Brunelli Cecilia, Fumagalli Marco, Magnavacca Andrea, Martinelli Giulia, Colombo Francesca, Casiraghi Antonella, Melzi Gloria, Marabini Laura, Restani Patrizia, Dell'Agli Mario
Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, 20133 Milan, Italy.
Department of Pharmaceutical Sciences, Università degli Studi di Milano, 20133 Milan, Italy.
Antioxidants (Basel). 2019 May 16;8(5):134. doi: 10.3390/antiox8050134.
Psoriasis is a chronic cutaneous condition characterized by the release of pro-inflammatory mediators and oxidative stress. The reduction of these factors is currently the most effective strategy to inhibit the symptoms of pathology. Antioxidants from natural sources are increasingly used to improve skin conditions. Dried red leaves from grapevine ( L., cv Teinturiers) showed anti-inflammatory and anti-bacterial activities, but their possible effects on keratinocytes have not been previously investigated. In this study we tested the ability of a water extract from grapevine leaves (VVWE) to inhibit inflammatory conditions in human keratinocytes (HaCaT cells), challenged with proinflammatory (tumor necrosis factor-α (TNF-α) or lipopolysaccharide (LPS)) or prooxidant (ultraviolet B radiation (UVB) or HO) mediators. VVWE inhibited interleukin-8 (IL-8) secretion induced by proinflammatory stimuli, acting on the IL-8 promoter activity, but the effect was lower when prooxidant mediators were used. The effect was partly explained by the reduction of nuclear factor-κB (NF-κB)-driven transcription and nuclear translocation. Furthermore, vascular endothelial growth factor (VEGF) secretion, a regulator of angiogenesis, was inhibited by VVWE, but not matrix metalloproteinase-9 (MMP-9), a protease involved in matrix remodeling. VVWE, assayed on Franz diffusion cell system, showed a marked reduction of High Performance Liquid Chromatography (HPLC)-identified compounds. Pure molecules individually failed to reduce TNF-α-induced IL-8 release, suggesting synergistic effects or the presence of other bioactive compounds still unknown.
银屑病是一种慢性皮肤病，其特征是促炎介质的释放和氧化应激。减少这些因素是目前抑制病理症状的最有效策略。天然来源的抗氧化剂越来越多地用于改善皮肤状况。葡萄（L.，品种：Teinturiers）的干红叶子具有抗炎和抗菌活性，但它们对角质形成细胞的可能影响此前尚未进行研究。在本研究中，我们测试了葡萄叶水提取物（VVWE）抑制人角质形成细胞（HaCaT细胞）炎症状态的能力，这些细胞受到促炎介质（肿瘤坏死因子-α（TNF-α）或脂多糖（LPS））或促氧化介质（紫外线B辐射（UVB）或过氧化氢（HO））的刺激。VVWE通过作用于白细胞介素-8（IL-8）启动子活性，抑制促炎刺激诱导的IL-8分泌，但在使用促氧化介质时效果较低。这种作用部分是由于核因子-κB（NF-κB）驱动的转录和核转位减少所致。此外，血管内皮生长因子（VEGF）的分泌（一种血管生成调节剂）受到VVWE的抑制，但基质金属蛋白酶-9（MMP-9，一种参与基质重塑的蛋白酶）未受抑制。在Franz扩散池系统上测定的VVWE显示，高效液相色谱（HPLC）鉴定的化合物显著减少。单独的纯分子未能减少TNF-α诱导的IL-8释放，这表明存在协同作用或仍有其他未知的生物活性化合物。
