Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Mérida, México.
School of Food Science & Nutrition, University of Leeds, Leeds, UK.
J Food Sci. 2021 Jul;86(7):2861-2871. doi: 10.1111/1750-3841.15780. Epub 2021 Jun 2.
Salvia hispanica demonstrated to be a source of protein fractions with anticancer activity. The effect of the protein fractions <1, 1-3, and 3-5 kDa, obtained by ultrafiltration of the S. hispanica hydrolysate, was evaluated on the cellular viability of four cancer cell lines (MCF-7, Caco2, PC-3, and HepG2) and on human fibroblasts (hFB) at different concentrations (0.25, 0.5, 0.75, and 1 mg/ml). The protein fractions did not show cytotoxic effects on hFB. The protein fraction <1 kDa at 1 mg/ml showed the highest statistical effect on the cellular viability of all evaluated cancer lines; thus, its amino acid sequence was analyzed. From the multicriteria decision analysis of the peptide sequences obtained by mass spectrometry, the peptide KLKKNL with potential anticancer activity was selected. In conclusion, protein fractions could represent a therapeutic option for cancer treatment. However, further investigations are necessary to establish conclusive arguments. PRACTICAL APPLICATION: The work of this article is based on the background of the increasing potential of peptides for the treatment of chronic diseases. The results of this study present peptides of low molecular weight, obtained from chia seeds, as a potential adjuvant option for cancer treatment.
芝麻菜被证明是具有抗癌活性的蛋白质片段的来源。通过对芝麻菜水解物进行超滤，获得了<1、1-3 和 3-5 kDa 的蛋白质片段，并评估了它们在不同浓度（0.25、0.5、0.75 和 1 mg/ml）下对四种癌细胞系（MCF-7、Caco2、PC-3 和 HepG2）和人成纤维细胞（hFB）的细胞活力的影响。这些蛋白质片段对 hFB 没有细胞毒性作用。在 1 mg/ml 时，<1 kDa 的蛋白质片段对所有评估的癌细胞系的细胞活力表现出最高的统计学影响；因此，对其进行了氨基酸序列分析。通过质谱获得的肽序列的多标准决策分析，选择了具有潜在抗癌活性的肽 KLKKNL。总之，蛋白质片段可能成为癌症治疗的一种治疗选择。然而，需要进一步的研究来建立结论性的论点。实际应用：本文的工作基于肽在治疗慢性疾病方面的潜在应用不断增加的背景。本研究的结果提供了低分子量的肽，这些肽来自奇亚籽，可以作为癌症治疗的潜在辅助选择。
