Department of Biology, Faculty of Sciences and Arts Khulais, University of Jeddah, Saudi Arabia; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Microb Pathog. 2020 Oct;147:104384. doi: 10.1016/j.micpath.2020.104384. Epub 2020 Jul 15.
ZnO nanoparticles (ZnO-NPs) can be used as nano medicine for Staphylococcus aureus infection, which causes deleterious effects on liver, kidney and lung tissue, as it causes catarrhal bronchitis, peri-bronchial oedema, lymphocytic granulomas, oedematous fluid and haemorrhage inside the bronchi, and interstitial pneumonia. In this research ZnO nanoparticle (ZnO-NPs) synthesis by biogenic method using green alga Ulva fasciata and by wet chemical method. Both of them tested in vitro and in vivo against Staphylococcus aureus. The characterization of ZnO-NPs was detected by U.V spectroscopy, Fourier-transform infrared (FTIR), Energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM). In vivo assessment eight groups, each group contain of five rats and the treatment as follow (1) an uninfected control group; (2) an infected group; groups (3), (4), and (5) were injected with biogenic or chemical ZnO-NPs or zinc acetate, as the bulk group, respectively; and groups (6), (7) and (8) were infected and then treated in the same manner as groups (3), (4), and (5), respectively. The blood profile, biochemical parameters, phagocytic activity and histological assessment of liver, kidney and lung tissue of each rat was investigated after 20 days. The rats treated with 5 mg/1 kg natural ZnO-NPs showed improved lung characteristics, and the number of platelets in the infected groups treated with ZnO-NPs from chemical and natural sources (G6 and G7) was close to those in the control group. However, the trend was reversed for regarding lymphocytes, which remained at higher levels in uninfected animals treated with synthetic ZnO-NPs (G4) than in infected rats treated with synthetic ZnO-NPs (G7). Moreover, a significant difference in phagocytic activity was found among all groups compared to that of controls. Compared to control group rats (G1), uninfected rats injected with only natural ZnO-NPs (G3) showed a significant (P < 0.05) improvement in the phagocytic index. We propose that ZnO-NPs produced from natural sources are preferable to those produced from chemical sources for use as nano medicine for the treatment of S. aureus infection in albino rats.
氧化锌纳米粒子（ZnO-NPs）可用作治疗金黄色葡萄球菌感染的纳米药物，金黄色葡萄球菌会对肝、肾和肺组织造成有害影响，导致卡他性支气管炎、支气管周围水肿、淋巴细胞性肉芽肿、支气管内水肿和出血以及间质性肺炎。本研究采用生物法（绿藻石莼）和湿法化学法合成 ZnO 纳米粒子（ZnO-NPs）。体外和体内均检测了其对金黄色葡萄球菌的抑制作用。通过紫外光谱、傅里叶变换红外光谱（FTIR）、能谱（EDX）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对 ZnO-NPs 进行了表征。体内评估分为 8 组，每组包含 5 只大鼠，处理方法如下：（1）未感染对照组；（2）感染组；（3）、（4）和（5）组分别注射生物合成或化学 ZnO-NPs 或醋酸锌作为块状组；（6）、（7）和（8）组感染后，分别以与（3）、（4）和（5）组相同的方式进行处理。20 天后，检测每组大鼠的血液特征、生化参数、吞噬活性和肝、肾、肺组织的组织学评估。用 5mg/1kg 天然 ZnO-NPs 治疗的大鼠显示出改善的肺部特征，而用天然和化学来源的 ZnO-NPs（G6 和 G7）治疗的感染组中的血小板数量接近对照组。然而，对于淋巴细胞来说，情况正好相反，与感染的大鼠相比，用合成 ZnO-NPs（G4）治疗的未感染动物中的淋巴细胞水平仍然较高。此外，与对照组相比，所有组的吞噬活性都有显著差异。与对照组大鼠（G1）相比，仅用天然 ZnO-NPs 注射的未感染大鼠（G3）的吞噬指数显著（P<0.05）提高。我们提出，与化学来源的 ZnO-NPs 相比，天然来源的 ZnO-NPs 更适合作为纳米药物治疗白化大鼠的金黄色葡萄球菌感染。
