College of Food Science and Technology, Hebei Agricultural University, Baoding, PR China.
College of Food Science and Technology, Hebei Agricultural University, Baoding, PR China; Medical College, Nankai University, Tianjin, PR China.
Food Chem. 2020 Dec 15;333:127524. doi: 10.1016/j.foodchem.2020.127524. Epub 2020 Jul 10.
Semicarbazide (SEM) is a protein-bound nitrofurazone metabolite that is detrimental to human health. Therefore, to ensure food safety, it is necessary to detect SEM in food samples. To this end, we developed a novel electrochemical sensor to detect SEM by using a molecularly imprinted polymer (MIP) as the recognition element. Computer-aided molecular modelling was performed to guide the synthesis of the MIP, and subsequently, MIP/carboxylated single-walled carbon-nanotubes/chitosan (MIP/SWNTs-COOH/CS) was prepared as the sensing platform to develop the electrochemical sensor. The linear range of the sensor was 0.04-7.6 ng mL, with a detection limit of 0.025 ng mL. The sensor was successfully applied to detect SEM in four different real samples, with recoveries ranging from 83.16% to 93.40%. The results indicated that the fabricated electrochemical sensor can be widely applied to detect SEM in the environment and in agri-food products.
氨基脲（SEM）是一种与蛋白质结合的呋喃西林代谢物，对人体健康有害。因此，为了确保食品安全，有必要检测食品样品中的 SEM。为此，我们开发了一种新型电化学传感器，通过使用分子印迹聚合物（MIP）作为识别元件来检测 SEM。计算机辅助分子建模用于指导 MIP 的合成，随后制备 MIP/羧基单壁碳纳米管/壳聚糖（MIP/SWNTs-COOH/CS）作为传感平台来开发电化学传感器。该传感器的线性范围为 0.04-7.6ng mL，检测限为 0.025ng mL。该传感器成功应用于四种不同实际样品中 SEM 的检测，回收率在 83.16%至 93.40%之间。结果表明，所制备的电化学传感器可广泛应用于环境和农业食品产品中 SEM 的检测。
