Li Zhen, Tyler William A, Haydar Tarik F
Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA.
Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, MA, USA.
Curr Opin Genet Dev. 2020 Dec;65:138-143. doi: 10.1016/j.gde.2020.05.043. Epub 2020 Jul 14.
Modern RNA sequencing methods have greatly increased our understanding of the molecular fingerprint of neurons, astrocytes and oligodendrocytes throughout the central nervous system (CNS). Technical approaches with greater sensitivity and throughput have uncovered new connections between gene expression, cell biology, and ultimately CNS function. In recent years, single cell RNA-sequencing (scRNA-seq) has made a large impact on the neurosciences by enhancing the resolution of types of cells that make up the CNS and shedding light on their developmental trajectories and how their diversity is modified across species. Here we will review the advantages, innovations, and challenges of the single cell genomics era and highlight how it has impacted our understanding of neurodevelopment and neurological function.
现代RNA测序方法极大地增进了我们对整个中枢神经系统（CNS）中神经元、星形胶质细胞和少突胶质细胞分子指纹的理解。具有更高灵敏度和通量的技术方法揭示了基因表达、细胞生物学以及最终CNS功能之间的新联系。近年来，单细胞RNA测序（scRNA-seq）通过提高构成CNS的细胞类型分辨率、阐明其发育轨迹以及它们在不同物种间多样性如何改变，对神经科学产生了重大影响。在此，我们将回顾单细胞基因组学时代的优势、创新和挑战，并强调其如何影响了我们对神经发育和神经功能的理解。
