Inhibition of Binding to Porcine Intestinal Cells by a Wood-Derived Prebiotic.

Numerous serovars can cause disease and contamination of animal-produced foods. Oligosaccharide-rich products capable of blocking pathogen adherence to intestinal mucosa are attractive alternatives to antibiotics as these have potential to prevent enteric infections. Presently, a wood-derived prebiotic composed mainly of glucose-galactose-mannose-xylose oligomers was found to inhibit mannose-sensitive binding of select and strains when reacted with . Tests for the ability of the prebiotic to prevent binding of a green fluorescent protein (GFP)-labeled to intestinal porcine epithelial cells (IPEC-J2) cultured in vitro revealed that prebiotic-exposed GFP-labeled bound > 30% fewer individual IPEC-J2 cells than did GFP-labeled having no prebiotic exposure. Quantitatively, 90% fewer prebiotic-exposed GFP-labeled cells were bound per individual IPEC-J2 cell compared to non-prebiotic exposed GFP-labeled . Comparison of invasiveness of DT104 against IPEC-J2 cells revealed greater than a 90% decrease in intracellular recovery of prebiotic-exposed DT104 compared to non-exposed controls (averaging 4.4 ± 0.2 log CFU/well). These results suggest compounds within the wood-derived prebiotic bound to and -produced adhesions and in the case of , this adhesion-binding activity inhibited the binding and invasion of IPEC-J2 cells.