Rajani J, Dastar B, Samadi F, Karimi Torshizi M A, Abdulkhani A, Esfandyarpour S
a Department of Animal and Poultry Nutrition, Faculty of Animal Science , Gorgan University of Agricultural Sciences and Natural Resources , Gorgan , Iran.
b Department of Animal and Poultry Physiology, Faculty of Animal Science , Gorgan University of Agricultural Sciences and Natural Resources , Gorgan , Iran.
Br Poult Sci. 2016 Oct;57(5):682-692. doi: 10.1080/00071668.2016.1200013. Epub 2016 Sep 22.
An in vitro and in vivo study was conducted to evaluate the fermentability of isolated galactoglucomannan oligosaccharides (GGMs) and the influence of their feeding on shedding and colonisation of Salmonella typhimurium, growth performance and intestinal morphology in broiler chicks. The in vitro data demonstrated that three probiotic lactic acid bacteria namely Lactobacillus casei, L. plantarum and Enterococcus faecium were able to ferment the extracted oligosaccharides and other tested sugars on a basal de Man Rogosa Sharpe media free from carbohydrate. For the in vivo experiment, 144 one-d-old male Ross 308 broiler chicks were divided into 6 experimental treatments (with 4 replicates) including two positive and negative controls which received a basal maize-soybean diet without any additives, supplementation of three levels of isolated GGMs (0.1%, 0.2% and 0.3%) and a commercial mannanoligosaccharide (MOS) at 0.2% to the basal diet. All birds except those in the negative control group were challenged orally with 1 × 10 cfu of S. typhimurium at 3-d post-hatch. The results revealed that challenge with S. typhimurium resulted in a significant reduction in body weight gain, feed intake, villus height, villus height to crypt depth ratio and villus surface area in all of infected chicks. Birds that were given GGMs or MOS showed better growth performance, increased villus height and villus surface area and decreased S. typhimurium colonisation than the positive control birds. GGM at 0.2% level was more effective than the other treatments in improving growth rate as well as gut health of broiler chicks.
开展了一项体外和体内研究，以评估分离的半乳甘露葡聚糖寡糖（GGMs）的发酵性，以及其添加对肉鸡沙门氏菌的脱落和定植、生长性能及肠道形态的影响。体外数据表明，三种益生菌乳酸菌，即干酪乳杆菌、植物乳杆菌和粪肠球菌，能够在不含碳水化合物的基础德氏乳酸杆菌培养基上发酵提取的寡糖及其他测试糖类。对于体内实验，144只1日龄雄性罗斯308肉鸡被分为6个实验处理组（每组4个重复），包括两个阳性和阴性对照组，分别给予不含任何添加剂的基础玉米-大豆日粮、在基础日粮中添加三个水平的分离GGMs（0.1%、0.2%和0.3%）以及0.2%的商业甘露寡糖（MOS）。除阴性对照组外，所有雏鸡在孵化后第3天经口接种1×10⁸cfu鼠伤寒沙门氏菌。结果显示，鼠伤寒沙门氏菌攻毒导致所有感染雏鸡的体重增加、采食量、绒毛高度、绒毛高度与隐窝深度比值及绒毛表面积显著降低。给予GGMs或MOS的雏鸡比阳性对照组雏鸡表现出更好的生长性能、增加的绒毛高度和绒毛表面积以及减少的鼠伤寒沙门氏菌定植。0.2%水平的GGM在提高肉鸡生长速度以及肠道健康方面比其他处理更有效。
