Carsanba Erdem, Papanikolaou Seraphim, Fickers Patrick, Erten Huseyin
Food Engineering Department, Faculty of Agriculture, Cukurova University, 01330 Adana, Turkey.
Amyris Bioproducts Portugal, Unipessoal, Lda, 4169-005 Porto, Portugal.
Microorganisms. 2020 Jul 15;8(7):1054. doi: 10.3390/microorganisms8071054.
Oleaginous microorganisms, such as , accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL.
产油微生物，如 ，积累的脂质在食品生物技术或生物柴油合成中具有有趣的应用。酵母具有许多优点，如底物使用范围广且对极端条件具有耐受性，同时在几种培养条件下它能产生较高的脂质生产率。基于此假设，在本研究中，使用12种不同的 菌株在摇瓶培养中于氮限制条件下利用基于葡萄糖的培养基来研究微生物脂质的生产。测试了12种新分离的或属于官方培养物保藏中心的野生型或突变型 菌株，它们产生了适量的脂质（高达1.30 g/L）；在许多情况下，氮限制导致培养基中产生柠檬酸。脂质主要由C16和C18脂肪酸组成。微生物脂质的大多数脂肪酸是不饱和的，主要对应于油酸、棕榈酸和亚油酸。菌株H917和Po1dL大量产生亚麻酸（C18:3）（占干细胞重量（DCW）的10%至20%，重量/重量）。
