Mason D Y
J Clin Pathol. 1977 Jun;30(6):541-6. doi: 10.1136/jcp.30.6.541.
Samples from 49 cases of myeloproliferative diseases were tested by an immunocytochemical technique for leucocyte lysozyme and lactoferrin. The presence of these constituents in myeloid precursors from cases of acute and chronic myeloid leukaemia reflected the degree of cellular maturation, lysozyme appearing (as it does in normal myeloid cells) at the stage of primary granule production (in promyelocytes), while lactoferrin wad detectable only in more mature, secondary granule-containing myeloid cells. Auer rods stained positively for lysozyme, in keeping with their relationship to primary granules. Monocytes from five cases of leukaemia showing predominantly monocytic differentiation were indistinguishable from normal monocytes in their staining reactions for lysozyme despite the presence of raised serum and urinary lysozyme levels. In four cases of acute myeloid leukaemia circulating polymorphs deficient in lactoferrin were detected: in one of these cases a similar percentage of polymorphs was lysozyme negative.
采用免疫细胞化学技术对49例骨髓增殖性疾病样本进行白细胞溶菌酶和乳铁蛋白检测。急性和慢性髓性白血病病例的髓系前体细胞中这些成分的存在反映了细胞成熟程度，溶菌酶（如同在正常髓系细胞中一样）出现在初级颗粒产生阶段（早幼粒细胞），而乳铁蛋白仅在更成熟的、含有次级颗粒的髓系细胞中可检测到。奥氏小体溶菌酶染色呈阳性，这与它们和初级颗粒的关系相符。5例主要表现为单核细胞分化的白血病病例的单核细胞，尽管血清和尿液溶菌酶水平升高，但其溶菌酶染色反应与正常单核细胞无异。在4例急性髓性白血病中检测到循环中的多形核白细胞缺乏乳铁蛋白：其中1例中类似比例的多形核白细胞溶菌酶呈阴性。
