Microscopy Services Dähnhardt GmbH, Flintbek, Germany.
Translationszentrum Regenerative Therapien, Fraunhofer Institut für Silikatforschung ISC, Würzburg, Germany.
Skin Pharmacol Physiol. 2020;33(4):189-197. doi: 10.1159/000509121. Epub 2020 Jul 17.
An accelerated healing of superficial wounds was demonstrated in clinical trials with a topical comfrey preparation (Symphytum × uplandicum Nyman). The effect has previously not been examined in skin models.
An established in vitro model of epidermal cells with the typical strata was used for the observation of effects of applied substances on skin regeneration. Damage corresponding to a typical abrasion was created on day 1 by punching an opening into the epidermal fine structure down to the stratum basale. Samples were either untreated (controls) or exposed to comfrey cream on days 2, 3, 5, and 6. Tissue samples were taken for light and electron microscopy on days 1, 4, and 7.
Application of comfrey cream led to a quicker regeneration of skin cells and to an earlier differentiation of the cells towards a normal fine structure with a visible distinction of epidermal strata, keratin, and corneocyte formation within 4-7 days. The study covered the early days of skin regeneration and confirms the benefits observed in published clinical trials and non-interventional studies in patients with abrasions.
在临床研究中，一种局部使用的紫草制剂（筋骨草 × 高地）表现出了加速浅表伤口愈合的效果。此前，这种效果尚未在皮肤模型中进行过检验。
本研究使用了一种已建立的表皮细胞体外模型，该模型具有典型的表皮层结构，可用于观察应用物质对皮肤再生的影响。在第 1 天，通过在表皮细微结构上打孔，直至基底层，来模拟典型的擦伤损伤。样本分为未处理组（对照组）和紫草乳膏处理组，分别在第 2、3、5 和 6 天进行处理。在第 1、4 和 7 天分别采集组织样本进行光镜和电镜检查。
应用紫草乳膏可促进皮肤细胞更快地再生，并使细胞更早地向正常的细微结构分化，在 4-7 天内可观察到表皮层、角蛋白和角质形成细胞的形成有明显区别。该研究涵盖了皮肤再生的早期阶段，证实了在已发表的临床研究和非介入性研究中观察到的擦伤患者的益处。
