Katkova V I, Shanina S N
Institute of Geology of the Federal Research Center «Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences», Syktyvkar, Russia.
Sud Med Ekspert. 2020;63(4):30-33. doi: 10.17116/sudmed20206304130.
The purpose of work is to determine the ratio of amino acid enantimers in bioapatite of human teeth and the influence of pathological processes in periodontium on the degree of racemization of amino acids. Objects of research - enamel without signs of caries, root dentin of permanent teeth and enamel of human carious milk teeth (=16). Identification and determination of amino acid content in the samples was performed on a GC-17A gas chromatograph (Shimadzu, Chirasil-L-Val capillary column). D-forms were detected for three amino acids: alanine (Ala), aspartic (Asp) and glutamic (Glu) acids. Studies have shown the highest D/L Asp and Glu ratios for bioapatite enamel of tooth, removed due to pathological changes in periodontal tissues, and root dentin of the retained teeth. The ratio D/L Asp indicates the absence of relationship between the degree of racemization of aspartic acid in the teeth involved in pathological processes and biological age. Low D/L ratios of Asp, Glu and Ala are determined in teeth removed by orthodontic indications. It was noted that the use of racemization degree of aspartic acid in enamel and dentin bioapatite as one of the methods for assessing the exact biological age of unidentified remains is problematic without identifying inflammatory and degenerative processes in periodontal tissue.
这项工作的目的是确定人牙齿生物磷灰石中氨基酸对映体的比例，以及牙周组织病理过程对氨基酸消旋程度的影响。研究对象——无龋齿迹象的牙釉质、恒牙的牙根牙本质和人类患龋乳牙的牙釉质（=16）。使用GC - 17A气相色谱仪（岛津，Chirasil - L - Val毛细管柱）对样品中的氨基酸含量进行鉴定和测定。检测到三种氨基酸的D型：丙氨酸（Ala）、天冬氨酸（Asp）和谷氨酸（Glu）。研究表明，因牙周组织病理变化而拔除牙齿的牙釉质生物磷灰石以及留存牙齿的牙根牙本质中，D/L天冬氨酸和谷氨酸的比例最高。D/L天冬氨酸的比例表明，参与病理过程的牙齿中天冬氨酸的消旋程度与生物学年龄之间不存在关联。因正畸指征而拔除的牙齿中，天冬氨酸、谷氨酸和丙氨酸的D/L比例较低。值得注意的是，如果不识别牙周组织中的炎症和退行性过程，将牙釉质和牙本质生物磷灰石中天冬氨酸的消旋程度作为评估身份不明遗骸确切生物学年龄的方法之一是有问题的。
