Leone F, Ilardi I
Instituto di malattie infettive, Università La Sapienza, Policlinico Umberto I, Roma.
Quad Sclavo Diagn. 1988 Jan-Dec;24(1-4):141-4.
The usefulness of detecting specific IgM antibodies in the immunodiagnosis of toxoplasmosis is fraught with false positive and false negative results. For instance false positivity can be obtained when rheumatoid factor is present in the serum while false negativity can occur because competing levels of IgG antibodies. We report the results obtained by the use of a simplified chromatographic method, commercially available in kits, for the separation of IgM from IgG in the immunodiagnosis of toxoplasmosis. The 1564 sera tested by indirect immunofluorescent technique (IFA) showed that 119 (7.6%) were positive if the whole serum was tested and only 7 (0.4%) after the chromatographic separation. This suggests that the use of this method can notably reduce the number of false results in sera with high IFA titer of IgM to toxoplasma.
在弓形虫病免疫诊断中检测特异性IgM抗体的方法存在大量假阳性和假阴性结果。例如，当血清中存在类风湿因子时会出现假阳性，而由于IgG抗体水平的竞争则可能出现假阴性。我们报告了使用一种简化的色谱方法（试剂盒中有售）在弓形虫病免疫诊断中从IgG中分离IgM所获得的结果。通过间接免疫荧光技术（IFA）检测的1564份血清显示，若检测全血清，119份（7.6%）呈阳性，而经色谱分离后仅7份（0.4%）呈阳性。这表明使用该方法可显著减少弓形虫IgM的IFA滴度较高的血清中的假结果数量。
