Robert Stan S, Singh Surinder P, Zhou Xue-Rong, Petrie James R, Blackburn Susan I, Mansour Peter M, Nichols Peter D, Liu Qing, Green Allan G
Food Futures National Research Flagship.
Funct Plant Biol. 2005 Jul;32(6):473-479. doi: 10.1071/FP05084.
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are nutritionally important long-chain (≥ C) omega-3 polyunsaturated fatty acids (ω3 LC-PUFA) currently obtained mainly from marine sources. A set of genes encoding the fatty acid chain elongation and desaturation enzymes required for the synthesis of LC-PUFA from their C PUFA precursors was expressed seed-specifically in Arabidopsis thaliana. This resulted in the synthesis of DHA, the most nutritionally important ω3 LC-PUFA, for the first time in seed oils, along with its precursor EPA and the ω6 LC-PUFA arachidonic acid (ARA). The assembled pathway utilised Δ5 and Δ6 desaturases that operate on acyl-CoA substrates and led to higher levels of synthesis of LC-PUFA than previously reported with acyl-PC desaturases. This demonstrates the potential for development of land plants as alternative sources of DHA and other LC-PUFA to meet the growing demand for these nutrients.
二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）是营养上重要的长链（≥C）ω-3多不饱和脂肪酸（ω3 LC-PUFA），目前主要从海洋来源获取。一组编码从其C PUFA前体合成LC-PUFA所需的脂肪酸链延长和去饱和酶的基因在拟南芥中种子特异性表达。这首次在种子油中合成了营养上最重要的ω3 LC-PUFA——DHA，以及其前体EPA和ω6 LC-PUFA花生四烯酸（ARA）。组装的途径利用了作用于酰基辅酶A底物的Δ5和Δ6去饱和酶，并且与之前报道的酰基-PC去饱和酶相比，导致LC-PUFA的合成水平更高。这证明了陆地植物作为DHA和其他LC-PUFA替代来源以满足对这些营养素不断增长的需求的开发潜力。
