Graham Michelle A, Ramírez Mario, Valdés-López Oswaldo, Lara Miguel, Tesfaye Mesfin, Vance Carroll P, Hernandez Georgina
USDA-ARS, Corn Insects and Crop Genetics Research Unit, Ames, IA 50010, USA.
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A Cuernavaca, Mor. México.
Funct Plant Biol. 2006 Aug;33(8):789-797. doi: 10.1071/FP06101.
Common bean (Phaseolus vulgaris L.) is the world's most important grain legume for direct human consumption. However, the soils in which common bean predominate are frequently limited by the availability of phosphorus (P). Improving bean yield and quality requires an understanding of the genes controlling P acquisition and use, ultimately utilising these genes for crop improvement. Here we report an in silico approach for the identification of genes involved in adaptation of P. vulgaris and other legumes to P-deficiency. Some 22 groups of genes from four legume species and Arabidopsis thaliana, encoding diverse functions, were identified as statistically over-represented in EST contigs from P-stressed tissues. By combining bioinformatics analysis with available micro / macroarray technologies and clustering results across five species, we identified 52 P. vulgaris candidate genes belonging to 19 categories as induced by P-stress response. Transport-related, stress (defence and regulation) signal transduction genes are abundantly represented. Manipulating these genes through traditional breeding methodologies and / or biotechnology approaches may allow us to improve crop P-nutrition.
普通菜豆（Phaseolus vulgaris L.）是全球最重要的供人类直接食用的食用豆类。然而，普通菜豆种植为主的土壤常常受到磷（P）有效性的限制。提高菜豆产量和品质需要了解控制磷吸收和利用的基因，最终利用这些基因来改良作物。在此，我们报告一种计算机模拟方法，用于鉴定参与普通菜豆和其他豆科植物适应缺磷的基因。从四种豆科植物和拟南芥中鉴定出约22组具有不同功能的基因，这些基因在缺磷胁迫组织的EST重叠群中在统计学上过度富集。通过将生物信息学分析与现有的微阵列/宏阵列技术以及五个物种的聚类结果相结合，我们鉴定出52个属于19类的普通菜豆候选基因，这些基因由磷胁迫响应诱导。与转运相关、应激（防御和调节）信号转导基因大量存在。通过传统育种方法和/或生物技术方法操纵这些基因可能使我们能够改善作物的磷营养状况。
